ArticlePloS one2015
Vitamin C Compound Mixtures Prevent Ozone-Induced Oxidative Damage in Human Keratinocytes as Initial Assessment of Pollution Protection.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 91 citations in OpenAlex.
- Efficacy of Procyanidins against In Vivo Cellular Oxidative Damage: A Systematic Review and Meta-Analysis.PloS one · 2015Pooled it
- Air Pollution and Skin Diseases.Annals of dermatology · 2025Review
- ROS and calcium signaling are critical determinant of skin pigmentation.Cell calcium · 2025Review
- Vγ9Vδ2 T cells expanded with vitamin C combined with HMBPPFrontiers in cellular and infection microbiology · 2025Article
- Dietary Modulation of the Immune System.Nutrients · 2024Review
- Cutaneous Redox Senescence.Biomedicines · 2024Review
- Environmental Air Pollutants Affecting Skin Functions with Systemic Implications.International journal of molecular sciences · 2023Review
- Air Pollution: Possible Interaction between the Immune and Nervous System?International journal of environmental research and public health · 2022Review
- Ultraviolet Light Protection: Is It Really Enough?Antioxidants (Basel, Switzerland) · 2022Review
- Review
- Inflammasome involvement in CS-induced damage in HaCaT keratinocytes.In vitro cellular & developmental biology. Animal · 2022Article
- Modeling of Temporal Exposure to the Ambient Environment and Eczema Severity.JID innovations : skin science from molecules to population health · 2022Article
- Deferoxamine Treatment Improves Antioxidant Cosmeceutical Formulation Protection against Cutaneous Diesel Engine Exhaust Exposure.Antioxidants (Basel, Switzerland) · 2021Article
- Protective effects of a comprehensive topical antioxidant against ozone-induced damage in a reconstructed human skin model.Archives of dermatological research · 2021Article
- Bench approaches to study the detrimental cutaneous impact of tropospheric ozone.Journal of exposure science & environmental epidemiology · 2021Review
- Effects of Citrus Fruit Juices and Their Bioactive Components on Inflammation and Immunity: A Narrative Review.Frontiers in immunology · 2021Review
- Mechanism underlying the effect of SO2-induced oxidation on human skin keratinocytes.Medicine · 2020Article
- Evaluation of oxidative damage and Nrf2 activation by combined pollution exposure in lung epithelial cells.Environmental science and pollution research international · 2020Article
- Medaka (International journal of molecular sciences · 2020Article
- Additive effect of combined pollutants to UV induced skin OxInflammation damage. Evaluating the protective topical application of a cosmeceutical mixture formulation.Redox biology · 2020Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionOne of the main functions of cutaneous tissues is to protect our body from the outdoor insults. Ozone (O3) is among the most toxic stressors to which we are continuously exposed and because of its critical location, the skin is one of the most susceptible tissues to the oxidative damaging effect of O3. O3 is not able to penetrate the skin, and although it is not a radical per se, the damage is mainly a consequence of its ability to induce oxidative stress via the formation of lipid peroxidation products. AIM OF STUDY: In this study we investigated the protective effect of defined "antioxidant" mixtures against O3 induced oxidative stress damage in human keratinocytes and understand their underlying mechanism of action.
resultsResults showed that the mixtures tested were able to protect human keratinocytes from O3-induced cytotoxicity, inhibition of cellular proliferation, decrease the formation of HNE protein adducts, ROS, and carbonyls levels. Furthermore, we have observed the decreased activation of the redox sensitive transcription factor NF-kB, which is involved in transcribing pro-inflammatory cytokines and therefore constitutes one of the main players associated with O3 induced skin inflammation. Cells exposed to O3 demonstrated a dose dependent increase in p65 subunit nuclear expression as a marker of NF-kB activation, while pre-treatment with the mixtures abolished NF-kB nuclear translocation. In addition, a significant activation of Nrf2 in keratinocytes treated with the mixtures was also observed.
conclusionOverall this study was able to demonstrate a protective effect of the tested compounds versus O3-induced cell damage in human keratinocytes. Pre-treatment with the tested compounds significantly reduced the oxidative damage induced by O3 exposure and this protective effect was correlated to the abolishment of NF-kB nuclear translocation, as well as activation of Nrf2 nuclear translocation activating the downstream defence enzymes involved in cellular detoxification process.
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