ArticlePloS one2015
Bacterial Landscape of Bloodstream Infections in Neutropenic Patients via High Throughput Sequencing.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 42 citations in OpenAlex.
- Potentially pathogenic culturable bacteria in hemodialysis waters.BMC microbiology · 2024Article
- Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study.Journal of clinical medicine · 2023Article
- Pylephlebitis: A Systematic Review on Etiology, Diagnosis, and Treatment of Infective Portal Vein Thrombosis.Diagnostics (Basel, Switzerland) · 2023Review
- Article
- Plasma Microbial Cell-free DNA Next-generation Sequencing in the Diagnosis and Management of Febrile Neutropenia.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Infection prevention requirements for the medical care of immunosuppressed patients: recommendations of the Commission for Hospital Hygiene and Infection Prevention (KRINKO) at the Robert Koch Institute.GMS hygiene and infection control · 2022Article
- Next-Generation Sequencing to Detect Pathogens in Pediatric Febrile Neutropenia: A Single-Center Retrospective Study of 112 Cases.Open forum infectious diseases · 2021Article
- Article
- Tracking bacterial DNA patterns in septic progression using 16s rRNA gene amplicon sequencing analysis.International journal of clinical and experimental pathology · 2021Article
- Direct DNA Sequencing-Based Analysis of Microbiota Associated with Hematological Malignancies in the Eastern Province of Saudi Arabia.BioMed research international · 2021Article
- Microbiota changes in a pediatric acute lymphocytic leukemia mouse model.MicrobiologyOpen · 2020Article
- Clinical metagenomics for infectious corneal ulcers: Rags to riches?The ocular surface · 2020Article
- Comparison of Blood Bacterial Communities in Periodontal Health and Periodontal Disease.Frontiers in cellular and infection microbiology · 2020Article
- Assessment of a metabarcoding approach for the characterisation of vector-borne bacteria in canines from Bangkok, Thailand.Parasites & vectors · 2019Article
- The Not-so-Sterile Womb: Evidence That the Human Fetus Is Exposed to Bacteria Prior to Birth.Frontiers in microbiology · 2019Article
- Metagenome Analysis as a Tool to Study Bacterial Infection Associated with Acute Surgical Abdomen.Journal of clinical medicine · 2018Article
- Detection of pathogenic bacteria in the blood from sepsis patients using 16S rRNA gene amplicon sequencing analysis.PloS one · 2018Article
- Brain microbiota disruption within inflammatory demyelinating lesions in multiple sclerosis.Scientific reports · 2016Article
- Metagenomic analysis of bloodstream infections in patients with acute leukemia and therapy-induced neutropenia.Scientific reports · 2016Article
- Diagnosis of Bacterial Bloodstream Infections: A 16S Metagenomics Approach.PLoS neglected tropical diseases · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBloodstream infection (BSI) is a common and potentially life-threatening complication in patients with hematological malignancies and therapy-induced neutropenia. Administration of broad spectrum antibiotics has substantially decreased the mortality rate in febrile neutropenia, but bacterial infection is documented in only one-third or fewer of the cases. BSI is typically diagnosed by blood culture; however, this method can detect only culturable pathogens.
methodsIn the present study, a total of 130 blood samples from hematological patients receiving dose-intensive antitumoural treatment were subjected to 16S rRNA PCR and 62 of them were cultured. PCR positive samples were processed to high throughput sequencing by amplifying the V1-V3 regions of the 16S rRNA gene to obtain a full spectrum of bacteria present in BSI.
resultsFive phyla and 30 genera were identified with sequencing compared to 2 phyla and 4 genera with culture. The largest proportion of bacteria detected by sequencing belonged to Proteobacteria (55.2%), Firmicutes (33.4%) and Actinobacteria (8.6%), while Fusobacteria (0.4%) and Bacteroidetes (0.1%) were also detected. Ninety-eight percent of the bacteria identified by sequencing were opportunistic human pathogens and 65% belonged to the normal human microbiota.
conclusionsThe present study indicates that BSIs in neutropenic hosts contain a much broader diversity of bacteria, likely with host origin, than previously realized. The elevated ratio of Proteobacteria in BSI corroborates the results found in other systemic inflammatory diseases, such as inflammatory bowel disease or mucosal infections. This knowledge may become of value for tailoring antimicrobial drug administration.
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