Evidence map›Paper›PMID 26269578›Full record

ArticleMolecular biology of the cell2015

RdgB2 is required for dim-light input into intrinsically photosensitive retinal ganglion cells.

Marquis T Walker, Alan Rupp, Rebecca Elsaesser, Ali D Güler, Wenlong Sheng, Shijun Weng, David M Berson, Samer Hattar, Craig Montell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Emerging perspectives on multidomain phosphatidylinositol transfer proteins.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Marquis T WalkerDepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Alan RuppDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218.
Rebecca ElsaesserDepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
Ali D GülerDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218 Department of Biology, University of Virginia, Charlottesville, VA 22903.
Wenlong ShengInstitute of Neurobiology, Institute of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China.
Shijun WengInstitute of Neurobiology, Institute of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200032, China Department of Neuroscience, Brown University, Providence, RI 02912.
David M BersonDepartment of Neuroscience, Brown University, Providence, RI 02912.
Samer HattarDepartment of Biology, Johns Hopkins University, Baltimore, MD 21218 craig.montell@lifesci.ucsb.edu shattar@jhu.edu.
Craig MontellDepartment of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205 Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara CA 93106 craig.montell@lifesci.ucsb.edu shattar@jhu.edu.
Johns Hopkins University · USFudan University · CNBrown University · US

Funding

STRUCTURE AND FUNCTION OF MAMMALIAN GANGLION CELLSR01EY012793 · NEI · BROWN UNIVERSITY · PI BERSON, DAVID M. · 2000 to 2022
$9.3M
Unconventional roles for opsinsR01EY008117 · NEI · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI MONTELL, CRAIG · 1989 to 2022
$8.2M
TRP CHANNEL SIGNALING COMPLEX AND ITS ROLE IN VISIONR01EY010852 · NEI · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI MONTELL, CRAIG · 1995 to 2021
$7.9M
Role of mammalian retinal photoreceptors in non-image-forming visual functionsR01GM076430 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI ZHAO, HAIQING · 2005 to 2018
$4.8M
Biology of Photosensitive Ganglion CellsR01EY017137 · NEI · BROWN UNIVERSITY · PI BERSON, DAVID M. · 2006 to 2014
$2.9M
Molecular characterization of mouse melanopsin and second messenger pathway.R01EY019053 · NEI · UNIVERSITY OF MARYLAND BALTIMORE COUNTY · PI ROBINSON, PHYLLIS R · 2009 to 2012
$1.2M
NEI NIH HHS EY012793NEI NIH HHS EY017137NEI NIH HHS EY019053NEI NIH HHS EY08117NEI NIH HHS EY10852NEI NIH HHS R01 EY008117NEI NIH HHS R01 EY010852NEI NIH HHS R01 EY012793NEI NIH HHS R01 EY017137NEI NIH HHS R01 EY019053NIGMS NIH HHS GM076430NIGMS NIH HHS R01 GM076430
6 · The paper itself

Abstract

A subset of retinal ganglion cells is intrinsically photosensitive (ipRGCs) and contributes directly to the pupillary light reflex and circadian photoentrainment under bright-light conditions. ipRGCs are also indirectly activated by light through cellular circuits initiated in rods and cones. A mammalian homologue (RdgB2) of a phosphoinositide transfer/exchange protein that functions in Drosophila phototransduction is expressed in the retinal ganglion cell layer. This raised the possibility that RdgB2 might function in the intrinsic light response in ipRGCs, which depends on a cascade reminiscent of Drosophila phototransduction. Here we found that under high light intensities, RdgB2(-/-) mutant mice showed normal pupillary light responses and circadian photoentrainment. Consistent with this behavioral phenotype, the intrinsic light responses of ipRGCs in RdgB2(-/-) were indistinguishable from wild-type. In contrast, under low-light conditions, RdgB2(-/-) mutants displayed defects in both circadian photoentrainment and the pupillary light response. The RdgB2 protein was not expressed in ipRGCs but was in GABAergic amacrine cells, which provided inhibitory feedback onto bipolar cells. We propose that RdgB2 is required in a cellular circuit that transduces light input from rods to bipolar cells that are coupled to GABAergic amacrine cells and ultimately to ipRGCs, thereby enabling ipRGCs to respond to dim light.

Indexed as

AnimalsCalcium-Binding ProteinsCircadian RhythmEye ProteinsFemaleGene ExpressionLightLight Signal TransductionMaleMembrane Transport ProteinsMiceMice, KnockoutReflex, PupillaryRetinal Cone Photoreceptor CellsRetinal Ganglion CellsRetinal Rod Photoreceptor CellsCalcium-Binding ProteinsEye ProteinsMembrane Transport ProteinsPitpnm2 protein, mouse

Identifiers

PMID26269578
PMCPMC4603936
OpenAlexW1920137190

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.