Evidence map›Paper›PMID 26269569›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2015

Small-molecule-directed, efficient generation of retinal pigment epithelium from human pluripotent stem cells.

Julien Maruotti, Srinivas R Sripathi, Kapil Bharti, John Fuller, Karl J Wahlin, Vinod Ranganathan, Valentin M Sluch, Cynthia A Berlinicke, Janine Davis, Catherine Kim and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.

0numbers the graph read from it
0cells of the map it votes in
85citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

85 citing papers in PubMed.

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25 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Julien MaruottiWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Srinivas R SripathiWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Kapil BhartiUnit on Ocular and Stem Cell Translational Research, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;
John FullerWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Karl J WahlinWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Vinod RanganathanWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Valentin M SluchWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Cynthia A BerlinickeWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Janine DavisUnit on Ocular and Stem Cell Translational Research, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;
Catherine KimWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Lijun ZhaoWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Jun WanWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Jiang QianWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Barbara CorneoStem Cell Core Facility, Columbia University, New York, NY 10032;
Sally TempleNeural Stem Cell Institute, Rensselaer, NY 12144;
Ramin DubeyDepartment of Medicine, Stanford University School of Medicine, Stanford, CA 94305; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305;
Bogdan Z OlenyukDepartment of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90089;
Imran BhuttoWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Gerard A LuttyWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287;
Donald J ZackWilmer Eye Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287; Department of Molecular Biology and Genetics, Johns Hopkins University, Baltimore, MD 21287; Department of Neuroscience, Johns Hopkins University, Baltimore, MD 21287; Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287 dzack@jhmi.edu.

Funding

Developing autologous iPSC-RPE based IND for AMD patientsZIAEY000542 · NEI · NATIONAL EYE INSTITUTE · PI BHARTI, KAPIL · 2014 to 2025
$24.2M
BIOCHEMISTRY, CELLULAR AND MOLECULAR BIOLOGY PROGRAMT32GM007445 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI GREEN, RACHEL · 1985 to 2021
$24.1M
Wilmer Core Grant for Vision ResearchP30EY001765 · NEI · JOHNS HOPKINS UNIVERSITY · PI Jiang Qian · 1985 to 2026
$22.1M
Developing patient-specific iPS cell derived RPE disease modelsZIAEY000532 · NEI · NATIONAL EYE INSTITUTE · PI BHARTI, KAPIL · 2013 to 2025
$22.0M
Developing functional and authenticated RPE tissue from iPS cellsZIAEY000533 · NEI · NATIONAL EYE INSTITUTE · PI BHARTI, KAPIL · 2013 to 2025
$14.4M
A High Content Screening Approach for the Retinal Degenerative DiseasesR01EY024249 · NEI · JOHNS HOPKINS UNIVERSITY · PI BERLINICKE, CYNTHIA · 2015 to 2019
$2.0M
AMD THERAPY: A Screen for Molecules that Promote RPE Survival and DifferentiationR21EY023812 · NEI · JOHNS HOPKINS UNIVERSITY · PI ZACK, DONALD J. · 2013 to 2014
$441k
NEI NIH HHS 5R21EY023812-02NEI NIH HHS P30 EY001765NEI NIH HHS P30EY001765NEI NIH HHS R01 EY024249NEI NIH HHS R21 EY023812NIGMS NIH HHS T32 GM007445
6 · The paper itself

Abstract

Age-related macular degeneration (AMD) is associated with dysfunction and death of retinal pigment epithelial (RPE) cells. Cell-based approaches using RPE-like cells derived from human pluripotent stem cells (hPSCs) are being developed for AMD treatment. However, most efficient RPE differentiation protocols rely on complex, stepwise treatments and addition of growth factors, whereas small-molecule-only approaches developed to date display reduced yields. To identify new compounds that promote RPE differentiation, we developed and performed a high-throughput quantitative PCR screen complemented by a novel orthogonal human induced pluripotent stem cell (hiPSC)-based RPE reporter assay. Chetomin, an inhibitor of hypoxia-inducible factors, was found to strongly increase RPE differentiation; combination with nicotinamide resulted in conversion of over one-half of the differentiating cells into RPE. Single passage of the whole culture yielded a highly pure hPSC-RPE cell population that displayed many of the morphological, molecular, and functional characteristics of native RPE.

Indexed as

Cell DifferentiationHigh-Throughput Screening AssaysHumansPluripotent Stem CellsPolymerase Chain ReactionRetinal Pigment Epitheliumage-related macular degenerationdifferentiationhigh-throughput screeningpluripotent stem cellsretinal pigment epithelium

Identifiers

PMID26269569
PMCPMC4568212

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.