ArticlePlant physiology2015
Proteins of Unknown Biochemical Function: A Persistent Problem and a Roadmap to Help Overcome It.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- Limitations of current machine learning models in predicting enzymatic functions for uncharacterized proteins.G3 (Bethesda, Md.) · 2025Article
- Advancing plant biology through deep learning-powered natural language processing.Plant cell reports · 2024Review
- Phylogenomics of the Ecdysteroid Kinase-like (EcKL) Gene Family in Insects Highlights Roles in Both Steroid Hormone Metabolism and Detoxification.Genome biology and evolution · 2024Article
- Identification and preliminary characterization of conserved uncharacterized proteins fromPlant direct · 2023Article
- Activity-based annotation: the emergence of systems biochemistry.Trends in biochemical sciences · 2022Review
- Comparative Genomic Analysis of the DUF34 Protein Family Suggests Role as a Metal Ion Chaperone or Insertase.Biomolecules · 2021Article
- Characterization of thePlant signaling & behavior · 2021Article
- Mapping the plant proteome: tools for surveying coordinating pathways.Emerging topics in life sciences · 2021Review
- Identification and characterization of proteins of unknown function (PUFs) in Clostridium thermocellum DSM 1313 strains as potential genetic engineering targets.Biotechnology for biofuels · 2021Article
- A family of pathogen-induced cysteine-rich transmembrane proteins is involved in plant disease resistance.Planta · 2021Article
- Genetic Manipulation of Non-tuberculosis Mycobacteria.Frontiers in microbiology · 2021Review
- Current Advances in the Development of Decellularized Plant Extracellular Matrix.Frontiers in bioengineering and biotechnology · 2021Review
- Article
- Overexpression of a Novel Arabidopsis GeneFrontiers in plant science · 2020Article
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
- Ab initio GO-based mining for non-tandem-duplicated functional clusters in three model plant diploid genomes.PloS one · 2020Article
- Revolutionizing agriculture with synthetic biology.Nature plants · 2019Article
- Focus Issue Editorial: Synthetic Biology.Plant physiology · 2019Article
- Towards functional characterization of archaeal genomic dark matter.Biochemical Society transactions · 2019Review
- Toward a better understanding of folate metabolism in health and disease.The Journal of experimental medicine · 2019Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The number of sequenced genomes is rapidly increasing, but functional annotation of the genes in these genomes lags far behind. Even in Arabidopsis (Arabidopsis thaliana), only approximately 40% of enzyme- and transporter-encoding genes have credible functional annotations, and this number is even lower in nonmodel plants. Functional characterization of unknown genes is a challenge, but various databases (e.g. for protein localization and coexpression) can be mined to provide clues. If homologous microbial genes exist-and about one-half the genes encoding unknown enzymes and transporters in Arabidopsis have microbial homologs-cross-kingdom comparative genomics can powerfully complement plant-based data. Multiple lines of evidence can strengthen predictions and warrant experimental characterization. In some cases, relatively quick tests in genetically tractable microbes can determine whether a prediction merits biochemical validation, which is costly and demands specialized skills.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.