Evidence map›Paper›PMID 26269519›Full record

ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2015

Shear stress blunts tubuloglomerular feedback partially mediated by primary cilia and nitric oxide at the macula densa.

Lei Wang, Chunyu Shen, Haifeng Liu, Shaohui Wang, Xinshan Chen, Richard J Roman, Luis A Juncos, Yan Lu, Jin Wei, Jie Zhang and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 21 citations in OpenAlex.

  1. A new view of macula densa cell microanatomy.American journal of physiology. Renal physiology · 2021
    Article
  2. Review
  3. NaHCOHypertension (Dallas, Tex. : 1979) · 2019
    Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Lei WangDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
Chunyu ShenDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida; Department of Forensic Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and.
Haifeng LiuDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
Shaohui WangDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
Xinshan ChenDepartment of Forensic Pathology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; and.
Richard J RomanDepartment of Physiology/Pharmacology, University of Mississippi Medical Center, Jackson Mississippi.
Luis A JuncosDepartment of Physiology/Pharmacology, University of Mississippi Medical Center, Jackson Mississippi.
Yan LuDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida; Department of Physiology/Pharmacology, University of Mississippi Medical Center, Jackson Mississippi.
Jin WeiDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
Jie ZhangDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
Kay-Pong YipDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida;
Ruisheng LiuDepartment of Molecular Pharmacology and Physiology, University of South Florida College of Medicine, Tampa, Florida; ruisheng@health.usf.edu.
University of South Florida · USUniversity of Mississippi Medical Center · USFlorida College · USHuazhong University of Science and Technology · CN

Funding

STRUCTURAL VASCULAR ADAPTATION OF THE MICROCIRCULATIONP01HL051971 · NHLBI · UNIVERSITY OF MISSISSIPPI MEDICAL CENTER · PI HESTER, ROBERT L · 1993 to 2018
$39.4M
The role of leptin in autoimmune-associated hypertensionP20GM104357 · NIGMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI TAYLOR, ERIN BASSFORD · 2013 to 2022
$23.4M
20-HETE-TGF-beta in Hypertension-Induced Renal InjuryR37HL036279 · NHLBI · UNIVERSITY OF MISSISSIPPI MED CTR · PI ROMAN, RICHARD J. · 2005 to 2014
$4.0M
Renal microcirculation and hypertension induced renal injuryR01DK104184 · NIDDK · UNIVERSITY OF MISSISSIPPI MED CTR · PI ROMAN, RICHARD J. · 2014 to 2017
$1.8M
Tubuloglomerular feedback and salt-sensitive hypertensionR01DK098582 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI LIU, RUISHENG · 2013 to 2016
$1.3M
VASA RECTA HEMODYNAMICS IN GENETIC HYPERTENSIONR01HL036279 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI ROMAN, RICHARD J. · 1986 to 2004
$1.3M
Primary cilia and modulation of the renal microcirculationR01DK099276 · NIDDK · UNIVERSITY OF SOUTH FLORIDA · PI LIU, RUISHENG · 2014 to 2017
$980k
NHLBI NIH HHS HL-051971NHLBI NIH HHS HL-36279NHLBI NIH HHS P01 HL051971NHLBI NIH HHS R01 HL036279NHLBI NIH HHS R37 HL036279NIDDK NIH HHS DK-098582NIDDK NIH HHS DK-099276NIDDK NIH HHS DK-104184NIDDK NIH HHS R01 DK098582NIDDK NIH HHS R01 DK099276NIDDK NIH HHS R01 DK104184NIGMS NIH HHS GM-104357NIGMS NIH HHS P20 GM104357
6 · The paper itself

Abstract

The present study tested whether primary cilia on macula densa serve as a flow sensor to enhance nitric oxide synthase 1 (NOS1) activity and inhibit tubuloglomerular feedback (TGF). Isolated perfused macula densa was loaded with calcein red and 4,5-diaminofluorescein diacetate to monitor cell volume and nitric oxide (NO) generation. An increase in tubular flow rate from 0 to 40 nl/min enhanced NO production by 40.0 ± 1.2%. The flow-induced NO generation was blocked by an inhibitor of NOS1 but not by inhibition of the Na/K/2Cl cotransporter or the removal of electrolytes from the perfusate. NO generation increased from 174.8 ± 21 to 276.1 ± 24 units/min in cultured MMDD1 cells when shear stress was increased from 0.5 to 5.0 dynes/cm(2). The shear stress-induced NO generation was abolished in MMDD1 cells in which the cilia were disrupted using a siRNA to ift88. Increasing the NaCl concentration of the tubular perfusate from 10 to 80 mM NaCl in the isolated perfused juxtaglomerular preparation reduced the diameter of the afferent arteriole by 3.8 ± 0.1 μm. This response was significantly blunted to 2.5 ± 0.2 μm when dextran was added to the perfusate to increase the viscosity and shear stress. Inhibition of NOS1 blocked the effect of dextran on TGF response. In vitro, the effects of raising perfusate viscosity with dextran on tubular hydraulic pressure were minimized by reducing the outflow resistance to avoid stretching of tubular cells. These results suggest that shear stress stimulates primary cilia on the macula densa to enhance NO generation and inhibit TGF responsiveness.

Indexed as

Stress, MechanicalAnimalsCell CountCell LineCiliaFeedback, PhysiologicalHydrogen-Ion ConcentrationKidney GlomerulusKidney TubulesMaleNitric OxideNitric Oxide Synthase Type IRabbitsRNA, Small InterferingViscosityNitric OxideNitric Oxide Synthase Type IRNA, Small Interferingmacula densanitric oxideprimary ciliatubuloglomerular feedback

Identifiers

PMID26269519
PMCPMC4666931
OpenAlexW1170360590

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.