ArticleNature communications2015
Slow-growing cells within isogenic populations have increased RNA polymerase error rates and DNA damage.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
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- A transient mutational burst occurs during yeast colony development.Molecular systems biology · 2025Article
- The impact of phenotypic heterogeneity on fungal pathogenicity and drug resistance.FEMS microbiology reviews · 2025Review
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- Genome instability footprint under rapamycin and hydroxyurea treatments.PLoS genetics · 2023Article
- Environmental Stability and Its Importance for the Emergence of Darwinian Evolution.Life (Basel, Switzerland) · 2023Article
- Genomic hallmarks and therapeutic implications of G0 cell cycle arrest in cancer.Genome biology · 2023Article
- Dynamic proteome trade-offs regulate bacterial cell size and growth in fluctuating nutrient environments.Communications biology · 2023Article
- Density fluctuations, homeostasis, and reproduction effects in bacteria.Communications biology · 2022Article
- A conserved expression signature predicts growth rate and reveals cell & lineage-specific differences.PLoS computational biology · 2021Article
- Microbial metabolic noise.WIREs mechanisms of disease · 2021Review
- High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression.Journal of visualized experiments : JoVE · 2021Article
- Insights on the Control of Yeast Single-Cell Growth Variability by Members of the Trehalose Phosphate Synthase (TPS) Complex.Frontiers in cell and developmental biology · 2021Article
- A Similar Speciation Process Relying on Cellular Stochasticity in Microbial and Cancer Cell Populations.iScience · 2020Review
- Chromatin Structure and Drug Resistance inJournal of fungi (Basel, Switzerland) · 2020Review
- Ethanol exposure increases mutation rate through error-prone polymerases.Nature communications · 2020Article
- Article
- Spontaneously slow-cycling subpopulations of human cells originate from activation of stress-response pathways.PLoS biology · 2019Article
- The Phenolic compound Kaempferol overcomes 5-fluorouracil resistance in human resistant LS174 colon cancer cells.Scientific reports · 2019Article
- Single cell functional genomics reveals the importance of mitochondria in cell-to-cell phenotypic variation.eLife · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Isogenic cells show a large degree of variability in growth rate, even when cultured in the same environment. Such cell-to-cell variability in growth can alter sensitivity to antibiotics, chemotherapy and environmental stress. To characterize transcriptional differences associated with this variability, we have developed a method--FitFlow--that enables the sorting of subpopulations by growth rate. The slow-growing subpopulation shows a transcriptional stress response, but, more surprisingly, these cells have reduced RNA polymerase fidelity and exhibit a DNA damage response. As DNA damage is often caused by oxidative stress, we test the addition of an antioxidant, and find that it reduces the size of the slow-growing population. More generally, we find a significantly altered transcriptome in the slow-growing subpopulation that only partially resembles that of cells growing slowly due to environmental and culture conditions. Slow-growing cells upregulate transposons and express more chromosomal, viral and plasmid-borne transcripts, and thus explore a larger genotypic--and so phenotypic--space.
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