Evidence map›Paper›PMID 26268925›Full record

ArticleInvestigational new drugs2015

Subcellular localization of anthracyclines in cultured rat cardiomyoblasts as possible predictors of cardiotoxicity.

Kazimierz Studzian, Krzysztof Kik, Malgorzata Lukawska, Irena Oszczapowicz, Malgorzata Strek, Leszek Szmigiero

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigational new drugs, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Kazimierz StudzianDepartment of Nucleic Acids Biochemistry, Medical University of Lodz, 251 Pomorska St., 92-213, Lodz, Poland.
Krzysztof KikDepartment of Nucleic Acids Biochemistry, Medical University of Lodz, 251 Pomorska St., 92-213, Lodz, Poland.
Malgorzata LukawskaInstitute of Biotechnology and Antibiotics, 5 Staroscinska St., 02-516, Warsaw, Poland.
Irena OszczapowiczInstitute of Biotechnology and Antibiotics, 5 Staroscinska St., 02-516, Warsaw, Poland.
Malgorzata StrekDepartment of Nucleic Acids Biochemistry, Medical University of Lodz, 251 Pomorska St., 92-213, Lodz, Poland.
Leszek SzmigieroDepartment of Nucleic Acids Biochemistry, Medical University of Lodz, 251 Pomorska St., 92-213, Lodz, Poland. leszek.szmigiero@umed.lodz.pl.
Medical University of Lodz · PLInstytut Biotechnologii i Antybiotyków · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we compared the cellular uptake, intracellular localization and cytotoxicity of two groups of anthracycline derivatives in cultured H9c2(2-1) rat cardiomyoblasts. The first group consisted of doxorubicin (DOX) and two of its derivatives containing a formamidino group (-N = CH-N<) at the C-3' position with a morpholine (DOXM) or a hexamethyleneimine (DOXH) ring. The second group consisted of daunorubicin (DRB) and its derivatives containing a morpholine (DRBM) or a hexamethyleneimine (DRBH) ring. DOXH and DRBH were taken up by cardiomyoblasts more efficiently than estimated for other tested anthracyclines. The cellular uptakes of DOXM and DRBM were reduced compared to those of the parent compounds. Applied structural modifications of DOX and DRB influenced the subcellular localization of the tested derivatives. DOX and DOXH were localized primarily in nuclei, whereas the other anthracyclines were found in the nuclei and cytoplasm. The percentages of the compounds that accumulated in the nuclei were 80.2 and 54.2 % for DOX and DOXH, respectively. The lowest nuclear accumulation values were observed for DRBM (19.9 %), DRBH (21.9 %) and DOXM (23.7 %). The ability of anthracyclines to accumulate in the nuclei correlated with their DNA binding constants (r = 0.858, P = 0.029). A correlation was found between the accumulation of the tested anthracyclines in the nuclei of cardiomyoblasts and their cardiotoxicity in vivo, which was observed in our previous study. We suggest that cytotoxicity and the anthracycline accumulation level in the nuclei of cultured cardiomyoblasts could be used for early prediction of their cardiotoxicity.

Indexed as

AnimalsAnthracyclinesCardiotoxicityCell CycleCell NucleusDaunorubicinDNA-Binding ProteinsDoxorubicinMyoblasts, CardiacRatsStructure-Activity RelationshipAnthracyclinesDaunorubicinDNA-Binding ProteinsDoxorubicinCellular uptakeFormamidinoanthracyclinesRat cardiomyoblastsSubcellular localization

Identifiers

PMID26268925
PMCPMC4768221
OpenAlexW2152954961

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.