ArticleBMC microbiology2015
Evidence for the involvement of cofilin in Aspergillus fumigatus internalization into type II alveolar epithelial cells.
Article in BMC microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Systemic Aspergillosis in Dogs: A Historical and Current State-of-the-Art Review.Veterinary sciences · 2026Review
- Review
- Epithelial uptake leads to fungal killingiScience · 2024Article
- Pathogenicity and virulence ofVirulence · 2023Review
- Article
- Involvement of JNK signaling in Aspergillus fumigatus-induced inflammatory factors release in bronchial epithelial cells.Scientific reports · 2023Article
- Characterisation ofJournal of fungi (Basel, Switzerland) · 2021Article
- Evaluation of Stable LifeAct-mRuby2- and LAMP1-NeonGreen Expressing A549 Cell Lines for Investigation ofInternational journal of molecular sciences · 2021Article
- Mechanisms of fungal dissemination.Cellular and molecular life sciences : CMLS · 2021Review
- iTRAQ‑based proteomic analysis of the interaction of A549 human lung epithelial cells with Aspergillus fumigatus conidia.Molecular medicine reports · 2020Article
- Phagolysosomal Survival Enables Non-lytic Hyphal Escape and Ramification Through Lung Epithelium DuringFrontiers in microbiology · 2020Article
- It takes a village: Phagocytes play a central role in fungal immunity.Seminars in cell & developmental biology · 2019Review
- Microbial uptake by the respiratory epithelium: outcomes for host and pathogen.FEMS microbiology reviews · 2019Review
- Article
- Gliotoxin Induces Cofilin Phosphorylation to Promote Actin Cytoskeleton Dynamics and Internalization ofFrontiers in microbiology · 2019Article
- Lung colonization by Aspergillus fumigatus is controlled by ZNF77.Nature communications · 2018Article
- Role of actin depolymerizing factor cofilin in Aspergillus fumigatus oxidative stress response and pathogenesis.Current genetics · 2018Article
- Proteome analysis reveals global response to deletion of mrflbA in Monascus ruber.Journal of microbiology (Seoul, Korea) · 2018Article
- Anti-Aspergillus Activities of the Respiratory Epithelium in Health and Disease.Journal of fungi (Basel, Switzerland) · 2018Review
- Role of Downregulation and Phosphorylation of Cofilin in Polarized Growth, MpkA Activation and Stress Response ofFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe internalization of Aspergillus fumigatus into alveolar epithelial cells (AECs) is tightly controlled by host cellular actin dynamics, which require close modulation of the ADF (actin depolymerizing factor)/cofilin family. However, the role of cofilin in A. fumigatus internalization into AECs remains unclear.
resultsHere, we demonstrated that germinated A. fumigatus conidia were able to induce phosphorylation of cofilin in A549 cells during the early stage of internalization. The modulation of cofilin activity by overexpression, knockdown, or mutation of the cofilin gene in A549 cells decreased the efficacy of A. fumigatus internalization. Reducing the phosphorylation status of cofilin with BMS-5 (LIM kinase inhibitor) or overexpression of the slingshot phosphatases also impeded A. fumigatus internalization. Both the C. botulimun C3 transferase (a specific RhoA inhibitor) and Y27632 (a specific ROCK inhibitor) reduced the internalization of A. fumigatus and the level of phosphorylated cofilin. β-1,3-glucan (the major component of the conidial cell wall) and its host cell receptor dectin-1 did not seem to be associated with cofilin phosphorylation during A. fumigatus infection.
conclusionThese results indicated that cofilin might be involved in the modulation of A. fumigatus internalization into type II alveolar epithelial cells through the RhoA-ROCK-LIM kinase pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.