ArticlePlant biotechnology journal2016
Co-expression of tonoplast Cation/H(+) antiporter and H(+)-pyrophosphatase from xerophyte Zygophyllum xanthoxylum improves alfalfa plant growth under salinity, drought and field conditions.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 127 citations in OpenAlex.
- Identification and characterization of salt-, drought-, and combined salt-drought stress-responsivePlant signaling & behavior · 2026Article
- Proteome-Transcriptome Discordance in Rice Under Drought Is Modulated by Post-Translational Modifications with Functional Consequences for Photosynthesis and Energy Metabolism.Plants (Basel, Switzerland) · 2026Article
- Genome wide identification, evolution and expression analysis of NHX gene family in cultivated barley (Hordeum vulgare. L) grown under salinity.Molecular biology reports · 2026Article
- Integrated ion, hormone, and proteomic analyses reveal the mechanisms of drought resistance in alfalfa (Medicago sativa).BMC plant biology · 2026Article
- Genome-wide identification and expression profiling of the NHX gene family in oat (Avena sativa L.).BMC genomics · 2026Article
- ZxNHX1 from a xerophyte outperforms AtNHX1 in sequestering NaPlant biotechnology journal · 2025Article
- Systematic identification, evolution and expression analyses of Brachypodium distachyon NHX gene family in response to salinity.Molecular biology reports · 2025Article
- Genome-wide identification and expression pattern of the phytochrome-interacting factors (PIFs) family in three alfalfa varieties.BMC genomics · 2025Article
- Molecular Mechanisms of Alfalfa Response to Abiotic Stresses.Plants (Basel, Switzerland) · 2025Review
- Thriving in a salty future: morpho-anatomical, physiological and molecular adaptations to salt stress in alfalfa (Medicago sativa L.) and other crops.Annals of botany · 2024Review
- Phosphorus fertilization enhanced overwintering, root system and forage yield of late-seeded alfalfa in sodic soils.Scientific reports · 2024Article
- Comparative Physiological and Gene Expression Analyses Reveal Mechanisms Involved in Maintaining Photosynthesis Capacity, Alleviating Ion Toxicity and Oxidative Stress of Kentucky Bluegrass under NaCl Treatment.Plants (Basel, Switzerland) · 2024Article
- Genome-wide identification of JAZ gene family members in autotetraploid cultivated alfalfa (Medicago sativa subsp. sativa) and expression analysis under salt stress.BMC genomics · 2024Article
- Physiological and Transcriptional Analyses Provide Insight into Maintaining Ion Homeostasis of Sweet Sorghum under Salt Stress.International journal of molecular sciences · 2023Article
- Elucidation of Physiological, Transcriptomic and Metabolomic Salinity Response Mechanisms inPlants (Basel, Switzerland) · 2023Article
- Cucumis sativus PHLOEM PROTEIN 2-A1 like gene positively regulates salt stress tolerance in cucumber seedlings.Plant molecular biology · 2023Article
- Physiological and transcriptomic analyses provide insight into thermotolerance in desert plant Zygophyllum xanthoxylum.BMC plant biology · 2023Article
- Root rot a silent alfalfa killer in China: Distribution, fungal, and oomycete pathogens, impact of climatic factors and its management.Frontiers in microbiology · 2022Review
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6 authors at 3 institutions in 2 countries.
Funding
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Abstract
Salinity and drought are major environmental factors limiting the growth and productivity of alfalfa worldwide as this economically important legume forage is sensitive to these kinds of abiotic stress. In this study, transgenic alfalfa lines expressing both tonoplast NXH and H(+)-PPase genes, ZxNHX and ZxVP1-1 from the xerophyte Zygophyllum xanthoxylum L., were produced via Agrobacterium tumefaciens-mediated transformation. Compared with wild-type (WT) plants, transgenic alfalfa plants co-expressing ZxNHX and ZxVP1-1 grew better with greater plant height and dry mass under normal or stress conditions (NaCl or water-deficit) in the greenhouse. The growth performance of transgenic alfalfa plants was associated with more Na(+), K(+) and Ca(2+) accumulation in leaves and roots, as a result of co-expression of ZxNHX and ZxVP1-1. Cation accumulation contributed to maintaining intracellular ions homoeostasis and osmoregulation of plants and thus conferred higher leaf relative water content and greater photosynthesis capacity in transgenic plants compared to WT when subjected to NaCl or water-deficit stress. Furthermore, the transgenic alfalfa co-expressing ZxNHX and ZxVP1-1 also grew faster than WT plants under field conditions, and most importantly, exhibited enhanced photosynthesis capacity by maintaining higher net photosynthetic rate, stomatal conductance, and water-use efficiency than WT plants. Our results indicate that co-expression of tonoplast NHX and H(+)-PPase genes from a xerophyte significantly improved the growth of alfalfa, and enhanced its tolerance to high salinity and drought. This study laid a solid basis for reclaiming and restoring saline and arid marginal lands as well as improving forage yield in northern China.
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