ArticlePloS one2015
Induction of Neural Progenitor-Like Cells from Human Fibroblasts via a Genetic Material-Free Approach.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Application of Small Molecules in the Central Nervous System Direct Neuronal Reprogramming.Frontiers in bioengineering and biotechnology · 2022Review
- Review
- Generation of Porcine Induced Neural Stem Cells Using the Sendai Virus.Frontiers in veterinary science · 2021Article
- Robust and Reproducible Generation of Induced Neural Stem Cells from Human Somatic Cells by Defined Factors.International journal of stem cells · 2020Article
- How to reprogram human fibroblasts to neurons.Cell & bioscience · 2020Review
- Take the shortcut - direct conversion of somatic cells into induced neural stem cells and their biomedical applications.FEBS letters · 2019Review
- Evolving principles underlying neural lineage conversion and their relevance for biomedical translation.F1000Research · 2019Review
- Valproic Acid Enhances Reprogramming Efficiency and Neuronal Differentiation on Small Molecules Staged-Induction Neural Stem Cells: Suggested Role of mTOR Signaling.Frontiers in neuroscience · 2019Article
- Neural Stem Cell-Based Regenerative Approaches for the Treatment of Multiple Sclerosis.Molecular neurobiology · 2018Review
- Generation of dopamine neuronal-like cells from induced neural precursors derived from adult human cells by non-viral expression of lineage factors.Journal of stem cells & regenerative medicine · 2018Article
- Transient Activation of Reprogramming Transcription Factors Using Protein Transduction Facilitates Conversion of Human Fibroblasts Toward Cardiomyocyte-Like Cells.Molecular biotechnology · 2017Article
- Understanding Parkinson's Disease through the Use of Cell Reprogramming.Stem cell reviews and reports · 2017Review
- Direct reprogramming and biomaterials for controlling cell fate.Biomaterials research · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA number of studies generated induced neural progenitor cells (iNPCs) from human fibroblasts by viral delivering defined transcription factors. However, the potential risks associated with gene delivery systems have limited their clinical use. We propose it would be safer to induce neural progenitor-like cells from human adult fibroblasts via a direct non-genetic alternative approach. METHODOLOGY/PRINCIPAL
findingsHere, we have reported that seven rounds of TAT-SOX2 protein transduction in a defined chemical cocktail under a 3D sphere culture gradually morphed fibroblasts into neuroepithelial-like colonies. We were able to expand these cells for up to 20 passages. These cells could give rise to cells that expressed neurons and glia cell markers both in vitro and in vivo. CONCLUSIONS/SIGNIFICANCE: These results show that our approach is beneficial for the genetic material-free generation of iNPCs from human fibroblasts where small chemical molecules can provide a valuable, viable strategy to boost and improve induction in a 3D sphere culture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.