ArticleMolecular metabolism2015
Knockout of STAT3 in skeletal muscle does not prevent high-fat diet-induced insulin resistance.
Article in Molecular metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Sex-specific role of high-fat diet and stress on behavior, energy metabolism, and the ventromedial hypothalamus.Biology of sex differences · 2024Article
- Dietary delivery of glycomacropeptide within the whey protein matrix is not effective in mitigating tissue ceramide deposition and obesity in mice fed a high-fat diet.Journal of dairy science · 2024Article
- Restoring glucose balance: Conditional HMGB1 knockdown mitigates hyperglycemia in a Streptozotocin induced mouse model.Heliyon · 2024Article
- Sexually dimorphic role of diet and stress on behavior, energy metabolism, and the ventromedial hypothalamus.bioRxiv : the preprint server for biology · 2023Article
- Rescue of Comorbid Behavioral and Metabolic Phenotypes of Arrhythmic Mice by Restoring CircadianBiological psychiatry global open science · 2023Article
- Antimicrobial Peptide LCN2 Inhibited UropathogenicInternational journal of molecular sciences · 2022Article
- Insulin Downregulated the Infection of UropathogenicMicroorganisms · 2021Article
- Article
- Stat3 activation induces insulin resistance via a muscle-specific E3 ubiquitin ligase Fbxo40.American journal of physiology. Endocrinology and metabolism · 2020Article
- Skeletal muscle mitochondrial function and exercise capacity are not impaired in mice with knockout of STAT3.Journal of applied physiology (Bethesda, Md. : 1985) · 2019Article
- Germline or inducible knockout of p300 or CBP in skeletal muscle does not alter insulin sensitivity.American journal of physiology. Endocrinology and metabolism · 2019Article
- Defining the contribution of skeletal muscle pyruvate dehydrogenase α1 to exercise performance and insulin action.American journal of physiology. Endocrinology and metabolism · 2018Article
- Denervation-activated STAT3-IL-6 signalling in fibro-adipogenic progenitors promotes myofibres atrophy and fibrosis.Nature cell biology · 2018Article
- Induced Cre-mediated knockdown of Brca1 in skeletal muscle reduces mitochondrial respiration and prevents glucose intolerance in adult mice on a high-fat diet.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- High Fat With High Sucrose Diet Leads to Obesity and Induces Myodegeneration.Frontiers in physiology · 2018Review
- Overload-mediated skeletal muscle hypertrophy is not impaired by loss of myofiber STAT3.American journal of physiology. Cell physiology · 2017Article
- Skeletal muscle inflammation and insulin resistance in obesity.The Journal of clinical investigation · 2017Review
- STAT3 in the systemic inflammation of cancer cachexia.Seminars in cell & developmental biology · 2016Review
- Signal transducer and activator of transcription 3 signaling as a potential target to treat muscle wasting diseases.Current opinion in clinical nutrition and metabolic care · 2016Review
- Muscle-specific inflammation induced by MCP-1 overexpression does not affect whole-body insulin sensitivity in mice.Diabetologia · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveIncreased signal transducer and activator of transcription 3 (STAT3) signaling has been implicated in the development of skeletal muscle insulin resistance, though its contribution, in vivo, remains to be fully defined. Therefore, the aim of this study was to determine whether knockout of skeletal muscle STAT3 would prevent high-fat diet (HFD)-induced insulin resistance.
methodsWe used Cre-LoxP methodology to generate mice with muscle-specific knockout (KO) of STAT3 (mKO). Beginning at 10 weeks of age, mKO mice and their wildtype/floxed (WT) littermates either continued consuming a low fat, control diet (CON; 10% of calories from fat) or were switched to a HFD (60% of calories from fat) for 20 days. We measured body composition, energy expenditure, oral glucose tolerance and in vivo insulin action using hyperinsulinemic-euglycemic clamps. We also measured insulin sensitivity in isolated soleus and extensor digitorum longus muscles using the 2-deoxy-glucose (2DOG) uptake technique.
resultsSTAT3 protein expression was reduced ∼75-100% in muscle from mKO vs. WT mice. Fat mass and body fat percentage did not differ between WT and mKO mice on CON and were increased equally by HFD. There were also no genotype differences in energy expenditure or whole-body fat oxidation. As determined, in vivo (hyperinsulinemic-euglycemic clamps) and ex vivo (2DOG uptake), skeletal muscle insulin sensitivity did not differ between CON-fed mice, and was impaired similarly by HFD.
conclusionsThese results demonstrate that STAT3 activation does not underlie the development of HFD-induced skeletal muscle insulin resistance.
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