ArticleJournal of biological engineering2015
Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors.
Article in Journal of biological engineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Multidimensional strategy enables scalable metabolome diversity in microbial fermentations.Scientific reports · 2026Article
- Biomanufacturing Potential of Streamlined Cells.Biotechnology and bioengineering · 2025Article
- Microtiter Plate Cultivation Systems Enable Chemically Diverse Metabolic Footprints During Bacterial Natural Product Discovery.Biotechnology and bioengineering · 2025Article
- Sigma Factors as Potential Targets to Enhance Recombinant Protein Expression.Biotechnology and bioengineering · 2025Article
- Small-scale fed-batch cultivations of Vibrio natriegens: overcoming challenges for early process development.Bioprocess and biosystems engineering · 2025Article
- Metabolic studies of Ogataea polymorpha using nine different corn steep liquors.BMC biotechnology · 2025Article
- Unraveling the impact of pH, sodium concentration, and medium osmolality on Vibrio natriegens in batch processes.BMC biotechnology · 2024Article
- Diffusion-driven fed-batch fermentation in perforated ring flasks.Biotechnology letters · 2024Article
- Biofuser: a multi-source data fusion platform for fusing the data of fermentation process devices.Frontiers in digital health · 2024Article
- Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.Microbial cell factories · 2023Article
- Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate.BMC biotechnology · 2023Article
- Spotting priming-active compounds using parsley cell cultures in microtiter plates.BMC plant biology · 2023Article
- Improved Time Resolved KPI and Strain Characterization of Multiple Hosts in Shake Flasks Using Advanced Online Analytics and Data Science.Bioengineering (Basel, Switzerland) · 2022Article
- Optimization of the production process for the anticancer lead compound illudin M: improving titers in shake-flasks.Microbial cell factories · 2022Article
- Implementation of Perforated Concentric Ring Walls Considerably Improves Gas-Liquid Mass Transfer of Shaken Bioreactors.Frontiers in bioengineering and biotechnology · 2022Article
- High-throughput dilution-based growth method enables time-resolved exo-metabolomics of Pseudomonas putida and Pseudomonas aeruginosa.Microbial biotechnology · 2021Article
- Need for speed: evaluation of dilute and shoot-mass spectrometry for accelerated metabolic phenotyping in bioprocess development.Analytical and bioanalytical chemistry · 2021Article
Corrections and comments
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Authors and funding
10 authors.
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Abstract
backgroundConventional experiments in small scale are often performed in a 'Black Box' fashion, analyzing only the product concentration in the final sample. Online monitoring of relevant process characteristics and parameters such as substrate limitation, product inhibition and oxygen supply is lacking. Therefore, fully equipped laboratory-scale stirred tank bioreactors are hitherto required for detailed studies of new microbial systems. However, they are too spacious, laborious and expensive to be operated in larger number in parallel. Thus, the aim of this study is to present a new experimental approach to obtain dense quantitative process information by parallel use of two small-scale culture systems with online monitoring capabilities: Respiration Activity MOnitoring System (RAMOS) and the BioLector device.
resultsThe same 'mastermix' (medium plus microorganisms) was distributed to the different small-scale culture systems: 1) RAMOS device; 2) 48-well microtiter plate for BioLector device; and 3) separate shake flasks or microtiter plates for offline sampling. By adjusting the same maximum oxygen transfer capacity (OTRmax), the results from the RAMOS and BioLector online monitoring systems supplemented each other very well for all studied microbial systems (E. coli, G. oxydans, K. lactis) and culture conditions (oxygen limitation, diauxic growth, auto-induction, buffer effects).
conclusionsThe parallel use of RAMOS and BioLector devices is a suitable and fast approach to gain comprehensive quantitative data about growth and production behavior of the evaluated microorganisms. These acquired data largely reduce the necessary number of experiments in laboratory-scale stirred tank bioreactors for basic process development. Thus, much more quantitative information is obtained in parallel in shorter time.
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