ArticlePlant physiology2015
A Method of Accounting for Enzyme Costs in Flux Balance Analysis Reveals Alternative Pathways and Metabolite Stores in an Illuminated Arabidopsis Leaf.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 51 citations in OpenAlex.
- Cytosolic fructose - an underestimated player in the regulation of sucrose biosynthesis.BMC plant biology · 2025Article
- Non-canonical plant metabolism.Nature plants · 2025Review
- Biophysical modelling as a tool for advancing plant science and engineering.Frontiers in plant science · 2025Review
- Systems biology of plant metabolic interactions.Journal of biosciences · 2024Review
- Maximizing multi-reaction dependencies provides more accurate and precise predictions of intracellular fluxes than the principle of parsimony.PLoS computational biology · 2023Article
- Analysis of companion cell and phloem metabolism using a transcriptome-guided model of Arabidopsis metabolism.Plant physiology · 2023Article
- Accurate flux predictions using tissue-specific gene expression in plant metabolic modeling.Bioinformatics (Oxford, England) · 2023Article
- Reconstruction of a catalogue of genome-scale metabolic models with enzymatic constraints using GECKO 2.0.Nature communications · 2022Article
- Inactivation of mitochondrial complex I stimulates chloroplast ATPase in Physcomitrium patens.Plant physiology · 2021Article
- Environment-coupled models of leaf metabolism.Biochemical Society transactions · 2021Review
- The Complementary Roles of Chloroplast Cyclic Electron Transport and Mitochondrial Alternative Oxidase to Ensure Photosynthetic Performance.Frontiers in plant science · 2021Review
- Flexibility in the Energy Balancing Network of Photosynthesis Enables Safe Operation under Changing Environmental Conditions.Plants (Basel, Switzerland) · 2020Article
- Multi-tissue to whole plant metabolic modelling.Cellular and molecular life sciences : CMLS · 2020Review
- Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light.Plant physiology · 2019Article
- Improved chloroplast energy balance during water deficit enhances plant growth: more crop per drop.Journal of experimental botany · 2018Article
- A Dynamic Multi-Tissue Flux Balance Model Captures Carbon and Nitrogen Metabolism and Optimal Resource Partitioning During Arabidopsis Growth.Frontiers in plant science · 2018Article
- Coordinated regulation of photosynthetic and respiratory components is necessary to maintain chloroplast energy balance in varied growth conditions.Journal of experimental botany · 2017Article
- Reconstruction ofFrontiers in plant science · 2017Article
- Metabolic model of central carbon and energy metabolisms of growing Arabidopsis thaliana in relation to sucrose translocation.BMC plant biology · 2016Article
- In silico metabolic network analysis of Arabidopsis leaves.BMC systems biology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flux balance analysis of plant metabolism is an established method for predicting metabolic flux phenotypes and for exploring the way in which the plant metabolic network delivers specific outcomes in different cell types, tissues, and temporal phases. A recurring theme is the need to explore the flexibility of the network in meeting its objectives and, in particular, to establish the extent to which alternative pathways can contribute to achieving specific outcomes. Unfortunately, predictions from conventional flux balance analysis minimize the simultaneous operation of alternative pathways, but by introducing flux-weighting factors to allow for the variable intrinsic cost of supporting each flux, it is possible to activate different pathways in individual simulations and, thus, to explore alternative pathways by averaging thousands of simulations. This new method has been applied to a diel genome-scale model of Arabidopsis (Arabidopsis thaliana) leaf metabolism to explore the flexibility of the network in meeting the metabolic requirements of the leaf in the light. This identified alternative flux modes in the Calvin-Benson cycle revealed the potential for alternative transitory carbon stores in leaves and led to predictions about the light-dependent contribution of alternative electron flow pathways and futile cycles in energy rebalancing. Notable features of the analysis include the light-dependent tradeoff between the use of carbohydrates and four-carbon organic acids as transitory storage forms and the way in which multiple pathways for the consumption of ATP and NADPH can contribute to the balancing of the requirements of photosynthetic metabolism with the energy available from photon capture.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.