ArticleThe Journal of biological chemistry2015
Connexin Type and Fluorescent Protein Fusion Tag Determine Structural Stability of Gap Junction Plaques.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- dCas-Based Tools to Visualize Chromatin or Modify Epigenetic Marks at Specific Plant Genomic Loci.Methods in molecular biology (Clifton, N.J.) · 2025Review
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- Regulation of long-range BMP gradients and embryonic polarity by propagation of local calcium-firing activity.Nature communications · 2024Article
- Genetically encoded barcodes for correlative volume electron microscopy.Nature biotechnology · 2023Article
- Article
- AAV-BR1 targets endothelial cells in the retina to reveal their morphological diversity and to deliver Cx43.The Journal of comparative neurology · 2022Article
- Glioblastoma-Astrocyte Connexin 43 Gap Junctions Promote Tumor Invasion.Molecular cancer research : MCR · 2022Article
- A review of bioeffects induced by focused ultrasound combined with microbubbles on the neurovascular unit.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2022Article
- Cx43 carboxyl terminal domain determines AQP4 and Cx30 endfoot organization and blood brain barrier permeability.Scientific reports · 2021Article
- Tuning the electronic transition energy of indole via substitution: application to identify tryptophan-based chromophores that absorb and emit visible light.Physical chemistry chemical physics : PCCP · 2021Article
- Generation and Characterization of Immortalized Mouse Cortical Astrocytes From Wildtype and Connexin43 Knockout Mice.Frontiers in cellular neuroscience · 2021Article
- Src Regulation of Cx43 Phosphorylation and Gap Junction Turnover.Biomolecules · 2020Article
- Comparative Analysis of Cx31 and Cx43 in Differentiation-Competent Rodent Keratinocytes.Biomolecules · 2020Article
- The dynamic Nexus: gap junctions control protein localization and mobility in distinct and surprising ways.Scientific reports · 2020Article
- Gap junction protein Connexin-43 is a direct transcriptional regulator of N-cadherin in vivo.Nature communications · 2018Article
- Cysteine residues in the cytoplasmic carboxy terminus of connexins dictate gap junction plaque stability.Molecular biology of the cell · 2017Article
- Adenosine receptors regulate gap junction coupling of the human cerebral microvascular endothelial cells hCMEC/D3 by CaThe Journal of physiology · 2017Article
- Connexin30.3 is expressed in mouse embryonic stem cells and is responsive to leukemia inhibitory factor.Scientific reports · 2017Article
- Up-regulation of gap junction in peripheral blood T lymphocytes contributes to the inflammatory response in essential hypertension.PloS one · 2017Article
- Connexin 43 Mediates White Adipose Tissue Beiging by Facilitating the Propagation of Sympathetic Neuronal Signals.Cell metabolism · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Gap junctions (GJs) are made up of plaques of laterally clustered intercellular channels and the membranes in which the channels are embedded. Arrangement of channels within a plaque determines subcellular distribution of connexin binding partners and sites of intercellular signaling. Here, we report the discovery that some connexin types form plaque structures with strikingly different degrees of fluidity in the arrangement of the GJ channel subcomponents of the GJ plaque. We uncovered this property of GJs by applying fluorescence recovery after photobleaching to GJs formed from connexins fused with fluorescent protein tags. We found that connexin 26 (Cx26) and Cx30 GJs readily diffuse within the plaque structures, whereas Cx43 GJs remain persistently immobile for more than 2 min after bleaching. The cytoplasmic C terminus of Cx43 was required for stability of Cx43 plaque arrangement. We provide evidence that these qualitative differences in GJ arrangement stability reflect endogenous characteristics, with the caveat that the sizes of the GJs examined were necessarily large for these measurements. We also uncovered an unrecognized effect of non-monomerized fluorescent protein on the dynamically arranged GJs and the organization of plaques composed of multiple connexin types. Together, these findings redefine our understanding of the GJ plaque structure and should be considered in future studies using fluorescent protein tags to probe dynamics of highly ordered protein complexes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.