ArticleNucleic acids research2016
DNA binding fluorescent proteins for the direct visualization of large DNA molecules.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Rapid and Sensitive Detection of RNA Viruses through Imaging of Marker Molecules Derived from Designed Circular DNA Probes.Small methods · 2025Article
- Artificial Intelligence-Enhanced Analysis of Genomic DNA Visualized with Nanoparticle-Tagged Peptides under Electron Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Force-enhanced sensitive and specific detection of DNA-intercalative agents directly from microorganisms at single-molecule level.Nucleic acids research · 2024Article
- Scanning Electron Microscopy Imaging of Large DNA Molecules Using a Metal-Free Electro-Stain Composed of DNA-Binding Proteins and Synthetic Polymers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Origin of minicircular mitochondrial genomes in red algae.Nature communications · 2023Article
- Article
- 21 Fluorescent Protein-Based DNA Staining Dyes.Molecules (Basel, Switzerland) · 2022Article
- Identification of a Novel Cathelicidin from theToxins · 2020Article
- Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer.Scientific reports · 2019Article
- Shifting Retroviral Vector Integrations Away from Transcriptional Start Sites via DNA-Binding Protein Domain Insertion into Integrase.Molecular therapy. Methods & clinical development · 2019Article
- Article
- TAMRA-polypyrrole for A/T sequence visualization on DNA molecules.Nucleic acids research · 2018Article
- Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Fluorescent proteins that also bind DNA molecules are useful reagents for a broad range of biological applications because they can be optically localized and tracked within cells, or provide versatile labels for in vitro experiments. We report a novel design for a fluorescent, DNA-binding protein (FP-DBP) that completely 'paints' entire DNA molecules, whereby sequence-independent DNA binding is accomplished by linking a fluorescent protein to two small peptides (KWKWKKA) using lysine for binding to the DNA phosphates, and tryptophan for intercalating between DNA bases. Importantly, this ubiquitous binding motif enables fluorescent proteins (Kd = 14.7 μM) to confluently stain DNA molecules and such binding is reversible via pH shifts. These proteins offer useful robust advantages for single DNA molecule studies: lack of fluorophore mediated photocleavage and staining that does not perturb polymer contour lengths. Accordingly, we demonstrate confluent staining of naked DNA molecules presented within microfluidic devices, or localized within live bacterial cells.
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Registered trials
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