ArticleCytotechnology2016
Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid.
Article in Cytotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 51 citations in OpenAlex.
- Influence of silver nanoparticles on the in vitro growth, phenolic profile, antioxidant potential, enzyme inhibition, and essential oil composition of Clinopodium nepeta subsp. spruneri (Boiss.) Bartolucci & F. Conti.Scientific reports · 2025Article
- Review
- Precursors and elicitor induced enhancement of cell biomass and phenolic compounds in cell suspensions of Indian basil-Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Article
- Engineering Considerations to Produce Bioactive Compounds from Plant Cell Suspension Culture in Bioreactors.Plants (Basel, Switzerland) · 2021Review
- Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid.Antioxidants (Basel, Switzerland) · 2020Review
- Establishment of a Cell Suspension Culture ofPlants (Basel, Switzerland) · 2020Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vitro approach to the production of rosmarinic acid (RA), a medicinally important caffeic acid ester, in a cell suspension culture (CSC) of Satureja khuzistanica Jamzad (Lamiaceae) has been investigated for the first time. The CSC was established from friable calli derived from shoot tip explants in Gamborg's B5 liquid medium supplemented with 30 g/L sucrose, 20 mg/L L-glutamine, 200 mg/L casein hydrolysate, 5 mg/L benzyladenine (BA) and 1 mg/L indole-3-butyric acid (IBA). The effect of nitrogen source (KNO3 and (NH4)2SO4) and their different concentrations on the fresh and dry weight (g/L), as well as RA content (mg/g dry weight) were measured. CSC growth measurements indicated a maximum specific cell growth rate of 1.5/day, a doubling time of 7.6 days and a high percentage of cell viability (96.4 %) throughout the growth cycle. Maximum cell fresh weight (353.5 g/L), dry weight (19.7 g/L) and RA production (180.0 mg/g) were attained at day 21 of culture. Cell growth and RA content were affected by nitrogen deficiency. Media containing 8.3 mM of total nitrogen (¼ of B5 standard medium) led to a minimum cell fresh weight (243.0 g/L), dry weight (17.4 g/L) and RA content (38.0 mg/g) after 21 days. The established CSC provided useful material for further optimization experiments aimed at a large-scale production of RA.
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