ArticleInternational journal of molecular sciences2015
Validation of FRET Assay for the Screening of Growth Inhibitors of Escherichia coli Reveals Elongasome Assembly Dynamics.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
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- Peptidoglycan Endopeptidase PBP7 Facilitates the Recruitment of FtsN to the Divisome and Promotes Peptidoglycan Synthesis in Escherichia coli.Molecular microbiology · 2024Article
- The two-component system ChvGI maintains cell envelope homeostasis in Caulobacter crescentus.PLoS genetics · 2022Article
- A Dynamic Network of Proteins Facilitate Cell Envelope Biogenesis in Gram-Negative Bacteria.International journal of molecular sciences · 2021Review
- MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA.PLoS genetics · 2020Article
- Detection ofBio-protocol · 2019Article
- Superfolder mTurquoise2Molecular microbiology · 2019Article
- RodZ: a key-player in cell elongation and cell division inAIMS microbiology · 2019Review
- The transpeptidase PbpA and noncanonical transglycosylase RodA ofThe Journal of biological chemistry · 2018Article
- Host-Polarized Cell Growth in Animal Symbionts.Current biology : CB · 2018Article
- Detection of Protein Interactions in the Cytoplasm and Periplasm ofBio-protocol · 2018Article
- Article
- Illuminating Messengers: An Update and Outlook on RNA Visualization in Bacteria.Frontiers in microbiology · 2017Review
- Structural Insights into Protein-Protein Interactions Involved in Bacterial Cell Wall Biogenesis.Antibiotics (Basel, Switzerland) · 2016Review
- LipidII: Just Another Brick in the Wall?PLoS pathogens · 2015Review
- ZapG (YhcB/DUF1043), a novel cell division protein in gamma-proteobacteria linking the Z-ring to septal peptidoglycan synthesis.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The increase in antibiotic resistant bacteria demands the development of new antibiotics against preferably new targets. The common approach is to test compounds for their ability to kill bacteria or to design molecules that inhibit essential protein activities in vitro. In the first case, the mode of action of the drug is unknown and in the second case, it is not known whether the compound will pass the impermeable barrier of the bacterial envelope. We developed an assay that detects the target of a compound, as well as its ability to pass the membrane(s) simultaneously. The Escherichia coli cytoskeletal protein MreB recruits protein complexes (elongasomes) that are essential for cell envelope growth. An in cell Förster Resonance Energy Transfer (FRET) assay was developed to detect the interaction between MreB molecules and between MreB and the elongasome proteins RodZ, RodA and PBP2. Inhibition of the polymerization of MreB by S-(3,4-dichlorobenzyl) isothiourea (A22) or of the activity of PBP2 by mecilinam resulted in loss or reduction of all measured interactions. This suggests that the interactions between the elongasome proteins are governed by a combination of weak affinities and substrate availability. This validated in cell FRET assay can be used to screen for cell envelope growth inhibitors.
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