Evidence map›Paper›PMID 26261538›Full record

ArticleInternational journal of clinical and experimental pathology2015

Impact of E6-associated protein on the proliferation and invasion of prostate cancer cells in bone metastasis.

Liyan Zhang, Xiaoguang Hu, Jiying Chen, Guilian Fu

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Liyan ZhangDepartment of Orthopedics, General Hospital of Chinese PLA Beijing 100853, China ; First Department of Orthopaedics, Affiliated Hospital of Beihua University Jilin 132000, Jilin Province, China.
Xiaoguang HuFirst Department of Orthopaedics, Affiliated Hospital of Beihua University Jilin 132000, Jilin Province, China.
Jiying ChenDepartment of Orthopedics, General Hospital of Chinese PLA Beijing 100853, China.
Guilian FuDepartment of Molecular Biology of Beihua University Jilin 132013, Jilin Province, China.
Beihua University · CNChinese PLA General Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo understand E6 associated protein (E6-AP)'s influence on prostate cancer cell proliferation and infiltration, thus providing the theoretical basis for developing therapeutic drugs for prostate cancer metastasis to the bone.

methodsElectroporation was performed to introduce linear regulatory plasmid PrevTet-off-in and conjugative plasmid PrevTRE2-flag-E6AP into prostate cancer cell line to establish wild-type E6-AP over-expressing transgenic LNCaP cell line; Western blot assay was adopted to examine expression levels of E6-AP, mammalian target of rapamycin (mTOR), protein kinase B (Akt), and phosphoinositide 3-kinase (PI3K); PI3K inhibitor LY294002 was applied to all the cells and MTT assay was used to measure cell proliferation; Matrigel invasion chamber assay was adopted to detect cancer cell migration and invasion.

resultsStably transfected LNCaP cells that over expressed E6-AP had higher expression levels of PI3K, Akt, and mTOR than control LNCaP cells; MTT assay showed that E6-AP-LNCaP cells were more responsive to the inhibitory effect of LY294002; Matrigel invasion chamber assay revealed increased cell crawling and adhesiveness of E6-AP-LNCaP cells.

conclusionStable over-expression of E6-AP increases the proliferation and invasion of LNCaP cells.

Indexed as

Cell MovementCell ProliferationBone NeoplasmsCell Line, TumorEnzyme InductionGene Expression Regulation, NeoplasticHumansMaleNeoplasm InvasivenessPhosphatidylinositol 3-KinasePhosphoinositide-3 Kinase InhibitorsPhosphorylationProstatic NeoplasmsProtein Kinase InhibitorsProto-Oncogene Proteins c-aktSignal TransductionMTOR protein, humanPhosphatidylinositol 3-KinasePhosphoinositide-3 Kinase InhibitorsProtein Kinase InhibitorsProto-Oncogene Proteins c-aktTOR Serine-Threonine KinasesUBE3A protein, humanUbiquitin-Protein LigasesE6-APinvasionproliferationProstate cancer

Identifiers

PMID26261538
PMCPMC4525872
OpenAlexW2416661594

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.