Evidence map›Paper›PMID 26261332›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2015

Unbiased phosphoproteomic method identifies the initial effects of a methacrylic acid copolymer on macrophages.

Michael Dean Chamberlain, Laura A Wells, Alexandra Lisovsky, Hongbo Guo, Ruth Isserlin, Ilana Talior-Volodarsky, Redouan Mahou, Andrew Emili, Michael V Sefton

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 17 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Michael Dean ChamberlainInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Laura A WellsInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Alexandra LisovskyInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Hongbo GuoDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
Ruth IsserlinDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
Ilana Talior-VolodarskyInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Redouan MahouInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
Andrew EmiliDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
Michael V SeftonInstitute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada M5S 3G9 michael.sefton@utoronto.ca.
University of Toronto · CA

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

An unbiased phosphoproteomic method was used to identify biomaterial-associated changes in the phosphorylation patterns of macrophage-like cells. The phosphorylation differences between differentiated THP1 (dTHP1) cells treated for 10, 20, or 30 min with a vascular regenerative methacrylic acid (MAA) copolymer or a control methyl methacrylate (MM) copolymer were determined by MS. There were 1,470 peptides (corresponding to 729 proteins) that were differentially phosphorylated in dTHP1 cells treated with the two materials with a greater cellular response to MAA treatment. In addition to identifying pathways (such as integrin signaling and cytoskeletal arrangement) that are well known to change with cell-material interaction, previously unidentified pathways, such as apoptosis and mRNA splicing, were also discovered.

Indexed as

Gene Expression ProfilingAdsorptionApoptosis Regulatory ProteinsBiocompatible MaterialsCell Adhesion MoleculesCell Line, TumorCytoskeletal ProteinsHumansIntracellular Signaling Peptides and ProteinsMacrophagesMaterials TestingMembrane ProteinsMethylmethacrylateNeoplasm ProteinsPhosphoprotein PhosphatasesPhosphoproteinsApoptosis Regulatory ProteinsBiocompatible MaterialsCell Adhesion MoleculesCytoskeletal ProteinsIntracellular Signaling Peptides and ProteinsMembrane ProteinsMethylmethacrylateNeoplasm ProteinsPhosphoprotein PhosphatasesPhosphoproteinsPolymethacrylic AcidsProtein KinasesTranscription Factorsmacrophagematerial–cell interactionsmethacrylic acidphosphoproteomic

Identifiers

PMID26261332
PMCPMC4553830
OpenAlexW2142624712

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.