ArticleThe Journal of cell biology2015
Microtubule motors transport phagosomes in the RPE, and lack of KLC1 leads to AMD-like pathogenesis.
Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 88 citations in OpenAlex.
- Spatio-temporal mechanosensitive phagocytic response of retinal pigment epithelial cells to reversible changes in substrate stiffness.Acta biomaterialia · 2026Article
- Toward Organelle Motility as a Functional Biomarker of Retinal Pigment Epithelium Health.Translational vision science & technology · 2026Article
- A mutation inMolecular vision · 2026Article
- Lack of Pnpla2 Accelerates Progression of AMD-Like Features in Mice.Investigative ophthalmology & visual science · 2025Article
- Phagocytosis by the retinal pigment epithelium: New insights into polarized cell mechanics.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- The Importance of Differentiated RPE Cultures for studying Cell Biological Processes.Advances in experimental medicine and biology · 2025Review
- Visualization of Stress Fiber Formation Induced by Photodynamic Therapy with Porphylipoprotein.Nanomaterials (Basel, Switzerland) · 2024Article
- Blue Light-Induced Accelerated Formation of Melanolipofuscin-Like Organelles in Japanese Quail RPE Cells: An Electron Microscopic Study.Investigative ophthalmology & visual science · 2024Article
- Machine learning-based 3D segmentation of mitochondria in polarized epithelial cells.Mitochondrion · 2024Article
- Non-Neovascular Age-Related Macular Degeneration Assessment: Focus on Optical Coherence Tomography Biomarkers.Diagnostics (Basel, Switzerland) · 2024Review
- Causal association of obesity-related anthropometric traits with myopia and the mediating role of educational attainment: a Mendelian randomization study.International journal of ophthalmology · 2024Article
- Polphylipoprotein-induced autophagy mechanism with high performance in photodynamic therapy.Communications biology · 2023Article
- Timing of Phagosome Maturation Depends on Their Transport Switching from Actin to Microtubule Tracks.The journal of physical chemistry. B · 2023Article
- Expression of two major isoforms of MYO7A in the retina: Considerations for gene therapy of Usher syndrome type 1B.Vision research · 2023Article
- Optineurin tunes outside-in signaling to regulate lysosome biogenesis and phagocytic clearance in the retina.Current biology : CB · 2023Article
- Ex Vivo OCT-Based Multimodal Imaging of Human Donor Eyes for Research into Age-Related Macular Degeneration.Journal of visualized experiments : JoVE · 2023Article
- Transcriptomic Changes Predict Metabolic Alterations in LC3 Associated Phagocytosis in Aged Mice.International journal of molecular sciences · 2023Article
- The impact of early RPE cell junction loss on VEGF, Ang-2, and TIMP secretion in vitro.Molecular vision · 2023Article
- Revisiting the Daily Timing of POS Phagocytosis.Advances in experimental medicine and biology · 2023Article
- Spatiotemporal control of actomyosin contractility by MRCKβ signaling drives phagocytosis.The Journal of cell biology · 2022Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The degradation of phagosomes, derived from the ingestion of photoreceptor outer segment (POS) disk membranes, is a major role of the retinal pigment epithelium (RPE). Here, POS phagosomes were observed to associate with myosin-7a, and then kinesin-1, as they moved from the apical region of the RPE. Live-cell imaging showed that the phagosomes moved bidirectionally along microtubules in RPE cells, with kinesin-1 light chain 1 (KLC1) remaining associated in both directions and during pauses. Lack of KLC1 did not inhibit phagosome speed, but run length was decreased, and phagosome localization and degradation were impaired. In old mice, lack of KLC1 resulted in RPE pathogenesis that was strikingly comparable to aspects of age-related macular degeneration (AMD), with an excessive accumulation of RPE and sub-RPE deposits, as well as oxidative and inflammatory stress responses. These results elucidate mechanisms of POS phagosome transport in relation to degradation, and demonstrate that defective microtubule motor transport in the RPE leads to phenotypes associated with AMD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.