ArticleThe Journal of biological chemistry2015
Heterologous expression of moss light-harvesting complex stress-related 1 (LHCSR1), the chlorophyll a-xanthophyll pigment-protein complex catalyzing non-photochemical quenching, in Nicotiana sp.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- LHCSR1 Functions as a Dimmer Switch for Light Harvesting.The journal of physical chemistry letters · 2026Article
- Structure of a diatom photosystem II supercomplex containing a member of Lhcx family and dimeric FCPII.Science advances · 2023Article
- Recent advances in expression and purification strategies for plant made vaccines.Frontiers in plant science · 2023Review
- Structure of the stress-related LHCSR1 complex determined by an integrated computational strategy.Communications biology · 2022Article
- Plant-Based Systems for Vaccine Production.Methods in molecular biology (Clifton, N.J.) · 2022Article
- The photo-inhibition of camphor leaves (PeerJ · 2020Article
- Functional analysis of LHCSR1, a protein catalyzing NPQ in mosses, by heterologous expression in Arabidopsis thaliana.Photosynthesis research · 2019Article
- Microsecond and millisecond dynamics in the photosynthetic protein LHCSR1 observed by single-molecule correlation spectroscopy.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Genetic Engineering, Synthetic Biology and the Light Reactions of Photosynthesis.Plant physiology · 2019Review
- Plant-Made Bet v 1 for Molecular Diagnosis.Frontiers in plant science · 2019Article
- Photoprotective, excited-state quenching mechanisms in diverse photosynthetic organisms.The Journal of biological chemistry · 2018Review
- Functional modulation of LHCSR1 protein from Physcomitrella patens by zeaxanthin binding and low pH.Scientific reports · 2017Article
- Single-molecule spectroscopy of LHCSR1 protein dynamics identifies two distinct states responsible for multi-timescale photosynthetic photoprotection.Nature chemistry · 2017Article
- Identification of pH-sensing Sites in the Light Harvesting Complex Stress-related 3 Protein Essential for Triggering Non-photochemical Quenching in Chlamydomonas reinhardtii.The Journal of biological chemistry · 2016Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oxygenic photosynthetic organisms evolved mechanisms for thermal dissipation of energy absorbed in excess to prevent formation of reactive oxygen species. The major and fastest component, called non-photochemical quenching, occurs within the photosystem II antenna system by the action of two essential light-harvesting complex (LHC)-like proteins, photosystem II subunit S (PSBS) in plants and light-harvesting complex stress-related (LHCSR) in green algae and diatoms. In the evolutionary intermediate Physcomitrella patens, a moss, both gene products are active. These proteins, which are present in low amounts, are difficult to purify, preventing structural and functional analysis. Here, we report on the overexpression of the LHCSR1 protein from P. patens in the heterologous systems Nicotiana benthamiana and Nicotiana tabacum using transient and stable nuclear transformation. We show that the protein accumulated in both heterologous systems is in its mature form, localizes in the chloroplast thylakoid membranes, and is correctly folded with chlorophyll a and xanthophylls but without chlorophyll b, an essential chromophore for plants and algal LHC proteins. Finally, we show that recombinant LHCSR1 is active in quenching in vivo, implying that the recombinant protein obtained is a good material for future structural and functional studies.
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