Evidence map›Paper›PMID 26260244›Full record

ArticleNeuroscience research2015

Enhanced conversion of induced neuronal cells (iN cells) from human fibroblasts: Utility in uncovering cellular deficits in mental illness-associated chromosomal abnormalities.

Eleonora Passeri, Ashley M Wilson, Amedeo Primerano, Mari A Kondo, Srona Sengupta, Rupali Srivastava, Minori Koga, Cassandra Obie, Peter P Zandi, Fernando S Goes and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuroscience research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Article
  3. Precision Medicine in Rare Diseases.Diseases (Basel, Switzerland) · 2020
    Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Eleonora PasseriDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Ashley M WilsonDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Amedeo PrimeranoDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Mari A KondoDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Srona SenguptaDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Rupali SrivastavaDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Minori KogaDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Cassandra ObieInstitute of Genetic Medicine, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Peter P ZandiDepartment of Mental Health, Johns Hopkins School of Public Health, 624 N. Broadway, Baltimore, MD 21287, USA.
Fernando S GoesDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
David ValleInstitute of Genetic Medicine, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA.
Judith L RapoportNIMH, Branch of Child Psychiatry, 10 Center Drive, Bethesda, MD 20892, USA.
Akira SawaDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA. Electronic address: asawa1@jhmi.edu.
Shin-ichi KanoDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA. Electronic address: skano2@jhmi.edu.
Koko IshizukaDepartment of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, 600 N. Wolfe Street, Baltimore, MD 21287, USA. Electronic address: kishizu2@jhmi.edu.

Funding

Translational Research Central ServicesP30CA006973 · NCI · JOHNS HOPKINS UNIVERSITY · PI Rosalie Wollett · 1985 to 2026
$208.6M
Project 3P50MH094268 · NIMH · JOHNS HOPKINS UNIVERSITY · PI SAWA, AKIRA · 2011 to 2020
$19.9M
Research Center for Molecular Pathogenesis of SchizophreniaP20MH084018 · NIMH · JOHNS HOPKINS UNIVERSITY · PI KATSANIS, NICHOLAS · 2008 to 2010
$3.3M
Nuclear pathway of DISC1 in major mental illnesses: biochemistry and cell biologyR01MH069853 · NIMH · JOHNS HOPKINS UNIVERSITY · PI SAWA, AKIRA · 2004 to 2012
$3.2M
Oxidative stress and schizophrenia: combination of cell biology and brain imagingR01MH092443 · NIMH · JOHNS HOPKINS UNIVERSITY · PI SAWA, AKIRA · 2011 to 2015
$1.8M
Defining of Neurodevelopmental Pathways Regulated by Neuregulin- DISC1 InteractioRC1MH088753 · NIMH · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ANTON, EVA S, SAWA, AKIRA · 2009 to 2010
$933k
Role of glia and inflammation in altered synapse development in schizophreniaR00MH093458 · NIMH · JOHNS HOPKINS UNIVERSITY · PI KANO, SHINICHI · 2014 to 2016
$737k
Gene expression and functional studies from olfactory neurons of bipolar disorderR21MH096208 · NIMH · JOHNS HOPKINS UNIVERSITY · PI ISHIZUKA, KOKO · 2012 to 2013
$436k
PICK1 knockout mice: role for D-serine in neonatal forebrainsR21MH085226 · NIMH · JOHNS HOPKINS UNIVERSITY · PI SAWA, AKIRA · 2010 to 2011
$369k
Role of Glia and Inflammation in Altered Synapse Development in SchizophreniaK99MH093458 · NIMH · JOHNS HOPKINS UNIVERSITY · PI KANO, SHINICHI · 2011 to 2012
$144k
NCI NIH HHS P30 CA006973NIMH NIH HHS K99 MH093458NIMH NIH HHS MH-069853NIMH NIH HHS MH-084018NIMH NIH HHS MH-085226NIMH NIH HHS MH-092443NIMH NIH HHS MH-093458NIMH NIH HHS MH-094268NIMH NIH HHS MH-096208NIMH NIH HHS P20 MH084018NIMH NIH HHS P50 MH094268NIMH NIH HHS R00 MH093458NIMH NIH HHS R01 MH069853NIMH NIH HHS R01 MH092443NIMH NIH HHS R21 MH085226NIMH NIH HHS R21 MH096208NIMH NIH HHS RC1 MH088753
6 · The paper itself

Abstract

The novel technology of induced neuronal cells (iN cells) is promising for translational neuroscience, as it allows the conversion of human fibroblasts into cells with postmitotic neuronal traits. However, a major technical barrier is the low conversion rate. To overcome this problem, we optimized the conversion media. Using our improved formulation, we studied how major mental illness-associated chromosomal abnormalities may impact the characteristics of iN cells. We demonstrated that our new iN cell culture protocol enabled us to obtain more precise measurement of neuronal cellular phenotypes than previous iN cell methods. Thus, this iN cell culture provides a platform to efficiently obtain possible cellular phenotypes caused by genetic differences, which can be more thoroughly studied in research using other human cell models such as induced pluripotent stem cells.

Indexed as

Chromosome AberrationsAdolescentAdultAzacitidineCell Culture TechniquesCell DifferentiationCulture MediaFemaleFibroblastsHumansHydroxamic AcidsInduced Pluripotent Stem CellsMaleMiddle AgedNeural Stem CellsSchizophreniaAzacitidineCulture MediaHydroxamic Acidstrichostatin AValproic AcidChildhood-onset schizophreniaConversion rateCopy number variations (CNVs)FibroblastsiN cellsProcess lengthSchizophreniaTranslational research

Identifiers

PMID26260244
PMCPMC4774521

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.