Evidence map›Paper›PMID 26259139›Full record

ArticleAnesthesiology2015

MicroRNA-21 Mediates Isoflurane-induced Cardioprotection against Ischemia-Reperfusion Injury via Akt/Nitric Oxide Synthase/Mitochondrial Permeability Transition Pore Pathway.

Shigang Qiao, Jessica M Olson, Mark Paterson, Yasheng Yan, Ivan Zaja, Yanan Liu, Matthias L Riess, Judy R Kersten, Mingyu Liang, David C Warltier and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Anesthesiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed
6.2field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 63 citations in OpenAlex.

  1. Trial
  2. Trial
  3. Cardioprotective Strategies After Ischemia-Reperfusion Injury.American journal of cardiovascular drugs : drugs, devices, and other interventions · 2024
    Review
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  8. Longitudinal Impact of WTC Dust Inhalation on Rat Cardiac Tissue Transcriptomic Profiles.International journal of environmental research and public health · 2022
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Shigang Qiao *Department of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Jessica M Olson *Department of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Mark PatersonDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Yasheng YanDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Ivan ZajaDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Yanan LiuDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Matthias L RiessDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Judy R KerstenDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Mingyu LiangDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
David C WarltierDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Zeljko J BosnjakDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Zhi-Dong GeDepartment of Anesthesiology (S.Q., J.M.O., M.P., Y.Y., I.Z., Y.L., J.R.K., D.C.W., Z.J.B., Z.D.G.), Department of Physiology (J.M.O., M.L., Z.J.B.), and Department of Pharmacology and Toxicology (J.R.K., D.C.W.), Medical College of Wisconsin, Milwaukee, Wisconsin, and Department of Anesthesiology (M.L.R.), TVHS VA Medical Center and Vanderbilt University, Nashville, Tennessee.
Zero to Three · USMedical College of Wisconsin · US

Funding

Stem Cell CoreP01GM066730 · NIGMS · MEDICAL COLLEGE OF WISCONSIN · PI LIANG, MINGYU · 2003 to 2019
$23.6M
Reperfusion Injury Protection Strategies During Basic Life SupportR01HL123227 · NHLBI · UNIVERSITY OF MINNESOTA · PI YANNOPOULOS, DEMETRIS · 2013 to 2017
$5.1M
Mechanisms of photobiomodulation in myocardial reperfusion injuryR01HL098490 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI BIENENGRAEBER, MARTIN · 2010 to 2013
$1.5M
Genetic Mechanisms of Resistance against Cardiac PreconditioningIK2BX001278 · VA · CLEMENT J. ZABLOCKI VA MEDICAL CENTER · PI RIESS, MATTHIAS L. · 2011 to 2014
–
BLRD VA IK2 BX001278NHLBI NIH HHS HL098490-03NHLBI NIH HHS HL123227-01NHLBI NIH HHS R01 HL098490NHLBI NIH HHS R01 HL123227NIGMS NIH HHS P01 GM066730NIGMS NIH HHS P01GM 066730
6 · The paper itself

Abstract

backgroundThe role of microRNA-21 in isoflurane-induced cardioprotection is unknown. The authors addressed this issue by using microRNA-21 knockout mice and explored the underlying mechanisms.

methodsC57BL/6 and microRNA-21 knockout mice were echocardiographically examined. Mouse hearts underwent 30 min of ischemia followed by 2 h of reperfusion in vivo or ex vivo in the presence or absence of 1.0 minimum alveolar concentration of isoflurane administered before ischemia. Cardiac Akt, endothelial nitric oxide synthase (eNOS), and neuronal nitric oxide synthase (nNOS) proteins were determined by Western blot analysis. Opening of the mitochondrial permeability transition pore (mPTP) in cardiomyocytes was induced by photoexcitation-generated oxidative stress and detected by rapid dissipation of tetramethylrhodamine ethyl ester fluorescence using a confocal microscope.

resultsGenetic disruption of miR-21 gene did not alter phenotype of the left ventricle, baseline cardiac function, area at risk, and the ratios of phosphorylated-Akt/Akt, phosphorylated-eNOS/eNOS, and phosphorylated-nNOS/nNOS. Isoflurane decreased infarct size from 54 ± 10% in control to 36 ± 10% (P < 0.05, n = 8 mice per group), improved cardiac function after reperfusion, and increased the ratios of phosphorylated-Akt/AKT, phosphorylated-eNOS/eNOS, and phosphorylated-nNOS/nNOS in C57BL/6 mice subjected to ischemia-reperfusion injury. These beneficial effects of isoflurane were lost in microRNA-21 knockout mice. There were no significant differences in time of the mPTP opening induced by photoexcitation-generated oxidative stress in cardiomyocytes isolated between C57BL/6 and microRNA-21 knockout mice. Isoflurane significantly delayed mPTP opening in cardiomyocytes from C57BL/6 but not from microRNA-21 knockout mice.

conclusionsIsoflurane protects mouse hearts from ischemia-reperfusion injury by a microRNA-21-dependent mechanism. The Akt/NOS/mPTP pathway is involved in the microRNA-21-mediated protective effect of isoflurane.

Indexed as

AnimalsCardiotonic AgentsCells, CulturedIsofluraneMaleMiceMice, Inbred C57BLMice, KnockoutMicroRNAsMitochondrial Membrane Transport ProteinsMitochondrial Permeability Transition PoreMyocardial Reperfusion InjuryMyocytes, CardiacNitric Oxide SynthaseOrgan Culture TechniquesProto-Oncogene Proteins c-aktCardiotonic AgentsIsofluraneMicroRNAsMIRN21 microRNA, mouseMitochondrial Membrane Transport ProteinsMitochondrial Permeability Transition PoreNitric Oxide SynthaseProto-Oncogene Proteins c-akt

Identifiers

PMID26259139
PMCPMC4573345
OpenAlexW1918744139

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.