ArticlePloS one2015
Application of β-lactamase reporter fusions as an indicator of effector protein secretion during infections with the obligate intracellular pathogen Chlamydia trachomatis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 56 citations in OpenAlex.
- Methods for Identifying Type III Secretion System Effectors in Chlamydia trachomatis.Methods in molecular biology (Clifton, N.J.) · 2027Article
- ThemBio · 2026Article
- Evidence for a post-invasion role of themBio · 2025Article
- Experimental Approaches to Visualize Effector Protein Translocation During Host-Pathogen Interactions.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- A molecular toolkit for heterologous protein secretion across Bacteroides species.Nature communications · 2024Article
- A molecular toolkit for heterologous protein secretion acrossbioRxiv : the preprint server for biology · 2023Article
- Type III Secretion inMicrobiology and molecular biology reviews : MMBR · 2023Review
- Identification and Preliminary Characterization of Novel Type III Secreted Effector Proteins in Chlamydia trachomatis.Infection and immunity · 2023Article
- Virulence Protein Pgp3 Is Insufficient To Mediate Plasmid-Dependent Infectivity of Chlamydia trachomatis.Infection and immunity · 2023Article
- Molecular pathogenesis ofFrontiers in cellular and infection microbiology · 2023Review
- Plasmid-mediated virulence inFrontiers in cellular and infection microbiology · 2023Review
- Article
- Recent advances in genetic systems in obligate intracellular human-pathogenic bacteria.Frontiers in cellular and infection microbiology · 2023Review
- GrgA overexpression inhibits Chlamydia trachomatis growth through sigmaMicrobial pathogenesis · 2021Article
- Inclusion Membrane Growth and Composition Are Altered by Overexpression of Specific Inclusion Membrane Proteins in Chlamydia trachomatis L2.Infection and immunity · 2021Article
- Got mutants? How advances in chlamydial genetics have furthered the study of effector proteins.Pathogens and disease · 2021Review
- Article
- Proximity Labeling To Map Host-Pathogen Interactions at the Membrane of a Bacterium-Containing Vacuole in Chlamydia trachomatis-Infected Human Cells.Infection and immunity · 2019Article
- The multiple functions of the numerousMicrobial cell (Graz, Austria) · 2019Review
- Structural basis for the homotypic fusion of chlamydial inclusions by the SNARE-like protein IncA.Nature communications · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Chlamydia spp. utilize multiple secretion systems, including the type III secretion system (T3SS), to deploy host-interactive effector proteins into infected host cells. Elucidation of secreted proteins has traditionally required ectopic expression in a surrogate T3SS followed by immunolocalization of endogenous candidate effectors to confirm secretion by chlamydiae. The ability to transform Chlamydia and achieve stable expression of recombinant gene products has enabled a more direct assessment of secretion. We adapted TEM-1 β-lactamase as a reporter system for assessment of chlamydial protein secretion. We provide evidence that this system facilitates visualization of secretion in the context of infection. Specifically, our findings provide definitive evidence that C. trachomatis CT695 is secreted during infection. Follow-up indirect immunofluorescence studies confirmed CT695 secretion and indicate that this effector can be secreted at multiple points during the chlamydial developmental cycle. Our results indicate that the BlaM-fusion reporter assay will allow efficacious identification of novel secreted proteins. Moreover, this approach can easily be adapted to enable more sophisticated studies of the secretion process in Chlamydia.
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