ArticleNature chemical biology2015
An excess of catalytically required motions inhibits the scavenger decapping enzyme.
Article in Nature chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Elucidation of the Catalytic Apparatus and Mechanism of Human Chitotriosidase‑1.ACS catalysis · 2025Article
- Analysis of ligand binding mechanism by dimeric receptors using stopped-flow fluorimetry-application to the human decapping scavenger enzyme.European biophysics journal : EBJ · 2025Article
- Article
- Multi-Site Conformational Exchange in the Synthetic Neomycin-Sensing Riboswitch Studied byAngewandte Chemie (International ed. in English) · 2023Article
- Assessing the applicability ofJournal of biomolecular NMR · 2023Article
- Observation of conformational changes that underlie the catalytic cycle of Xrn2.Nature chemical biology · 2022Article
- Targeted Degradation of mRNA Decapping Enzyme DcpS by a VHL-Recruiting PROTAC.ACS chemical biology · 2022Article
- Molecular basis of the selective processing of short mRNA substrates by the DcpS mRNA decapping enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Effect of the His-Tag Location on Decapping Scavenger Enzymes and Their Hydrolytic Activity toward Cap Analogs.ACS omega · 2020Article
- Modulation of conformational equilibrium by phosphorylation underlies the activation of deubiquitinase A.The Journal of biological chemistry · 2020Article
- Methyl-Based NMR Spectroscopy Methods for Uncovering Structural Dynamics in Large Proteins and Protein Complexes.Biochemistry · 2019Review
- Role of conformational dynamics in the evolution of novel enzyme function.Chemical communications (Cambridge, England) · 2018Review
- Label-free NMR-based dissociation kinetics determination.Journal of biomolecular NMR · 2017Article
- Role of Conformational Dynamics in the Evolution of Retro-Aldolase Activity.ACS catalysis · 2017Article
- Changes in conformational equilibria regulate the activity of the Dcp2 decapping enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The S. pombe mRNA decapping complex recruits cofactors and an Edc1-like activator through a single dynamic surface.RNA (New York, N.Y.) · 2016Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The scavenger decapping enzyme hydrolyzes the protective 5' cap structure on short mRNA fragments that are generated from the exosomal degradation of mRNAs. From static crystal structures and NMR data, it is apparent that the dimeric enzyme has to undergo large structural changes to bind its substrate in a catalytically competent conformation. Here we studied the yeast enzyme and showed that the associated opening and closing motions can be orders of magnitude faster than the catalytic turnover rate. This excess of motion is induced by the binding of a second ligand to the enzyme, which occurs at high substrate concentrations. We designed a mutant that disrupted the allosteric pathway that links the second binding event to the dynamics and showed that this mutant enzyme is hyperactive. Our data reveal a unique mechanism of substrate inhibition in which motions that are required for catalytic activity also inhibit efficient turnover when they are present in excess.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.