ArticleNature structural & molecular biology2015
Probing Gαi1 protein activation at single-amino acid resolution.
Article in Nature structural & molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Atomic, structural and molecular insights into GPCR ligand binding and signalling.Nature reviews. Molecular cell biology · 2026Review
- Ligand-dependent G protein dynamics underlying opioid efficacy.Nature structural & molecular biology · 2026Article
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- Ligand-dependent G protein dynamics underlying opioid signaling efficacy.bioRxiv : the preprint server for biology · 2025Article
- Computer-aided Drug Discovery Approaches in the Identification of Anticancer Drugs from Natural Products: A Review.Current computer-aided drug design · 2025Review
- Characterisation of aEndocrine oncology (Bristol, England) · 2025Article
- Agonist efficacy at the βFrontiers in pharmacology · 2025Article
- Structure of endothelin ETCommunications biology · 2024Article
- Active state structures of a bistable visual opsin bound to G proteins.Nature communications · 2024Article
- Distinct binding conformations of epinephrine with α- and β-adrenergic receptors.Experimental & molecular medicine · 2024Article
- Growth factor-dependent phosphorylation of GαScience signaling · 2024Article
- G Protein Activation Occurs via a Largely Universal Mechanism.The journal of physical chemistry. B · 2024Article
- Molecular annotation of G protein variants in a neurological disorder.Cell reports · 2023Article
- Molecular determinants of ligand efficacy and potency in GPCR signaling.Science (New York, N.Y.) · 2023Article
- Severity of GNAO1-Related Disorder Correlates with Changes in G-Protein Function.Annals of neurology · 2023Observational
- Structural aspects of rod opsin and their implication in genetic diseases.Pflugers Archiv : European journal of physiology · 2021Review
- Strategies towards Targeting Gαi/s Proteins: Scanning of Protein-Protein Interaction Sites To Overcome Inaccessibility.ChemMedChem · 2021Review
- A universal allosteric mechanism for G protein activation.Molecular cell · 2021Article
- Cryo-EM structure of an activated GPCR-G protein complex in lipid nanodiscs.Nature structural & molecular biology · 2021Article
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Authors and funding
11 authors.
Funding
Abstract
We present comprehensive maps at single-amino acid resolution of the residues stabilizing the human Gαi1 subunit in nucleotide- and receptor-bound states. We generated these maps by measuring the effects of alanine mutations on the stability of Gαi1 and the rhodopsin-Gαi1 complex. We identified stabilization clusters in the GTPase and helical domains responsible for structural integrity and the conformational changes associated with activation. In activation cluster I, helices α1 and α5 pack against strands β1-β3 to stabilize the nucleotide-bound states. In the receptor-bound state, these interactions are replaced by interactions between α5 and strands β4-β6. Key residues in this cluster are Y320, which is crucial for the stabilization of the receptor-bound state, and F336, which stabilizes nucleotide-bound states. Destabilization of helix α1, caused by rearrangement of this activation cluster, leads to the weakening of the interdomain interface and release of GDP.
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