ArticlePloS one2015
Transcriptomics Analysis of Crassostrea hongkongensis for the Discovery of Reproduction-Related Genes.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 77 citations in OpenAlex.
- Mapping molluscan endocrinology: a systematic and critical appraisal.Biological reviews of the Cambridge Philosophical Society · 2026Pooled it
- Bath-Immersion Delivery of nanos-MO-Vivo During Embryogenesis Perturbs Gonadal Development in the Pacific Oyster (Crassostrea gigas).Marine biotechnology (New York, N.Y.) · 2026Article
- Primordial germ cell specification and early developmental cell states in Pacific oyster.BMC genomics · 2025Article
- Comparative Transcriptomics Provides Insight into the Neuroendocrine Regulation of Spawning in the Black-Lip Rock Oyster (International journal of molecular sciences · 2025Article
- Deciphering the genetic basis of sex differentiation in silver-lipped pearl oyster (Zoological research · 2025Article
- Transcriptome sequencing of the endangered land snail Karaftohelix adamsi from the Island Ulleung: De novo assembly, annotation, valuation of fitness genes and SSR markers.Genes & genomics · 2024Article
- A review of the endangered mollusks transcriptome under the threatened species initiative of Korea.Genes & genomics · 2023Review
- Gender-Specific Metabolic Responses ofAntioxidants (Basel, Switzerland) · 2022Article
- Proteome and Transcriptome Analysis of Gonads Reveals Intersex in Gigantidas haimaensis.BMC genomics · 2022Article
- Article
- Integrated mRNA and miRNA expression profile analysis of female and male gonads in Hyriopsis cumingii.Scientific reports · 2021Article
- Comparative transcriptome analysis of three gonadal development stages reveals potential genes involved in gametogenesis of the fluted giant clam (Tridacna squamosa).BMC genomics · 2020Article
- Early embryonic exposure of freshwater gastropods to pharmaceutical 5-alpha-reductase inhibitors results in a surprising open-coiled "banana-shaped" shell.Scientific reports · 2019Article
- Transcriptome analysis of air-breathing land slug, Incilaria fruhstorferi reveals functional insights into growth, immunity, and reproduction.BMC genomics · 2019Article
- Putative Mitochondrial Sex Determination in the Bivalvia: Insights From a Hybrid Transcriptome Assembly in Freshwater Mussels.Frontiers in genetics · 2019Article
- Proteome and Transcriptome Analysis of Ovary, Intersex Gonads, and Testis Reveals Potential Key Sex Reversal/Differentiation Genes and Mechanism in Scallop Chlamys nobilis.Marine biotechnology (New York, N.Y.) · 2018Article
- Deciphering the Link between Doubly Uniparental Inheritance of mtDNA and Sex Determination in Bivalves: Clues from Comparative Transcriptomics.Genome biology and evolution · 2018Article
- FOXL2 and DMRT1L Are Yin and Yang Genes for Determining Timing of Sex Differentiation in the Bivalve MolluskFrontiers in physiology · 2018Article
- The Molecular Mechanism Underlying Pro-apoptotic Role of Hemocytes Specific Transcriptional Factor Lhx9 inFrontiers in physiology · 2018Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe reproductive mechanisms of mollusk species have been interesting targets in biological research because of the diverse reproductive strategies observed in this phylum. These species have also been studied for the development of fishery technologies in molluscan aquaculture. Although the molecular mechanisms underlying the reproductive process have been well studied in animal models, the relevant information from mollusks remains limited, particularly in species of great commercial interest. Crassostrea hongkongensis is the dominant oyster species that is distributed along the coast of the South China Sea and little genomic information on this species is available. Currently, high-throughput sequencing techniques have been widely used for investigating the basis of physiological processes and facilitating the establishment of adequate genetic selection programs.
resultsThe C.hongkongensis transcriptome included a total of 1,595,855 reads, which were generated by 454 sequencing and were assembled into 41,472 contigs using de novo methods. Contigs were clustered into 33,920 isotigs and further grouped into 22,829 isogroups. Approximately 77.6% of the isogroups were successfully annotated by the Nr database. More than 1,910 genes were identified as being related to reproduction. Some key genes involved in germline development, sex determination and differentiation were identified for the first time in C.hongkongensis (nanos, piwi, ATRX, FoxL2, β-catenin, etc.). Gene expression analysis indicated that vasa, nanos, piwi, ATRX, FoxL2, β-catenin and SRD5A1 were highly or specifically expressed in C.hongkongensis gonads. Additionally, 94,056 single nucleotide polymorphisms (SNPs) and 1,699 simple sequence repeats (SSRs) were compiled.
conclusionsOur study significantly increased C.hongkongensis genomic information based on transcriptomics analysis. The group of reproduction-related genes identified in the present study constitutes a new tool for research on bivalve reproduction processes. The large group of molecular markers discovered in this study will be useful for population screening and marker assisted selection programs in C.hongkongensis aquaculture.
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