ArticleAnalytical cellular pathology (Amsterdam)2015
Isolation and time lapse microscopy of highly pure hepatic stellate cells.
Article in Analytical cellular pathology (Amsterdam), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- Purification and Transcriptomic Characterization of Hypertrophied Hepatic Stellate Cells From CDAHFD Mouse Liver.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
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- The Impact of Toll-Like Receptor 5 on Liver Function in Age-Related Metabolic Disorders.Aging cell · 2025Article
- Isolation of Lung Retinoid-Containing Cells by Cell Sorting.Journal of visualized experiments : JoVE · 2025Article
- Exceptional Uptake, Limited Protein Expression: Liver Macrophages Lost in Translation of Synthetic mRNA.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Retinoids stored locally in the lung are required to attenuate the severity of acute lung injury in male mice.Nature communications · 2023Article
- Phalloidin Staining for F-Actin in Hepatic Stellate Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Isolation, Purification, and Culture of Primary Murine Hepatic Stellate Cells: An Update.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Five-in-One: Simultaneous isolation of multiple major liver cell types from livers of normal and NASH mice.Journal of cellular and molecular medicine · 2021Article
- Optimization of the isolation procedure and culturing conditions for hepatic stellate cells obtained from mouse.Bioscience reports · 2021Article
- Recent Advances in Practical Methods for Liver Cell Biology: A Short Overview.International journal of molecular sciences · 2020Review
- Celastrol exerts anti-inflammatory effect in liver fibrosis via activation of AMPK-SIRT3 signalling.Journal of cellular and molecular medicine · 2020Article
- Curcumin Recovers Intracellular Lipid Droplet Formation Through Increasing Perilipin 5 Gene Expression in Activated Hepatic Stellate Cells In Vitro.Current medical science · 2019Article
- Article
- The multikinase inhibitor regorafenib decreases angiogenesis and improves portal hypertension.Oncotarget · 2018Article
- Conditioned Medium from Human Amnion-Derived Mesenchymal Stem Cells Regulates Activation of Primary Hepatic Stellate Cells.Stem cells international · 2018Article
- Interaction between autophagy and senescence is required for dihydroartemisinin to alleviate liver fibrosis.Cell death & disease · 2017Article
- A method for high-throughput functional imaging of single cells within heterogeneous cell preparations.Scientific reports · 2016Article
- An Analysis of Monitoring Solutions for CAR T Cell Production.Healthcare technology lettersReview
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hepatic stellate cells (HSC) are the main effector cells for liver fibrosis. We aimed at optimizing HSC isolation by an additional step of fluorescence-activated cell sorting (FACS) via a UV laser. HSC were isolated from livers of healthy mice and animals subjected to experimental fibrosis. HSC isolation by iohexol- (Nycodenz) based density centrifugation was compared to a method with subsequent FACS-based sorting. We assessed cellular purity, viability, morphology, and functional properties like proliferation, migration, activation marker, and collagen expression. FACS-augmented isolation resulted in a significantly increased purity of stellate cells (>99%) compared to iohexol-based density centrifugation (60-95%), primarily by excluding doublets of HSC and Kupffer cells (KC). Importantly, this method is also applicable to young animals and mice with liver fibrosis. Viability, migratory properties, and HSC transdifferentiation in vitro were preserved upon FACS-based isolation, as assessed using time lapse microscopy. During maturation of HSC in culture, we did not observe HSC cell division using time lapse microscopy. Strikingly, FACS-isolated, differentiated HSC showed very limited molecular and functional responses to LPS stimulation. In conclusion, isolating HSC from mouse liver by additional FACS significantly increases cell purity by removing contaminations from other cell populations especially KC, without affecting HSC viability, migration, or differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.