Evidence map›Paper›PMID 26257991›Full record

ArticleAdipocyte

Optimization of in vitro cultivation strategies for human adipocyte derived stem cells.

K Storck, J Ell, S Regn, B Rittler-Ungetüm, H Mayer, T Schantz, D Müller, M Buchberger

Open access · goldAbstract read
In one paragraph

Article in Adipocyte. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

K StorckDepartment of Oto- Rhino- Laryngology; Head and Neck Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
J EllDepartment of Oto- Rhino- Laryngology; Head and Neck Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
S RegnDepartment of Oto- Rhino- Laryngology; Head and Neck Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
B Rittler-UngetümDepartment of Oto- Rhino- Laryngology; Head and Neck Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
H MayerDepartment of Oto- Rhino- Laryngology; Head and Neck Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
T SchantzDepartment of Plastic and Reconstructive Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
D MüllerDepartment of Plastic and Reconstructive Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
M BuchbergerDepartment of Oto- Rhino- Laryngology; Head and Neck Surgery; Klinikum Rechts der Isar der TU München ; Munich, Germany.
Klinikum rechts der Isar · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With adipose-derived stem cells being in the focus of research in regenerative medicine, the need arises for fast reliable cultivation protocols. We have tested the cultivation of human adipose-derived stem cells in endothelial cell growth medium prior to induction and differentiation, against the long-established use of DMEM/F12 medium-based cultivation protocols. We found that cultivation in endothelial cell growth medium not only accelerates growth before induction and differentiation, but also allows shorter induction and differentiation times than those following precultivation with DMEM/F12 medium with regard to the formation of mature adipocytes and to the viability undifferentiated cells. These results were first observed morphologically but could be confirmed by performing adiponectin ELISA and cell proliferation assays.

Indexed as

adipocyteADSCADSCs, Adipocyte Derived Stem CellsBM, Basal mediumcell-cultureculture mediumdifferentiationDM, Differentiation mediumFBS, Fetal Bovine SerumIM, Induction mediuminductionpreadipocyte

Identifiers

PMID26257991
PMCPMC4496977
OpenAlexW1982202168

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.