ArticleEvidence-based complementary and alternative medicine : eCAM2015
Gene Expression Response of Trichophyton rubrum during Coculture on Keratinocytes Exposed to Antifungal Agents.
Article in Evidence-based complementary and alternative medicine : eCAM, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Exploring the therapeutic potential and chemical properties of trans-chalcone: a comprehensive review.Future medicinal chemistry · 2024Pooled it
- Huangqin decoction disrupts energy metabolism of Trichophyton rubrum, with isocitrate lyase as a potential target.Scientific reports · 2026Article
- Biofilm development in three-dimensional models infected withMicrobiology spectrum · 2025Article
- Disclosing antifungal activity of Huangqin decoction upon Trichophyton mentagrophytes and exploring its potential inhibitory mechanisms through transcriptome sequencing and qRT-PCR.Scientific reports · 2025Article
- Synergistic activity between conventional antifungals and chalcone-derived compound against dermatophyte fungi and Candida spp.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Alternative splicing is a driving force that tunes metabolic adaptations to virulence traits in the dermatophyteFrontiers in cellular and infection microbiology · 2025Article
- Exploring CDKN1A Upregulation Mechanisms: Insights into Cell Cycle Arrest Induced by NC2603 Curcumin Analog in MCF-7 Breast Cancer Cells.International journal of molecular sciences · 2024Article
- The Transcriptome of BT-20 Breast Cancer Cells Exposed to Curcumin Analog NC2603 Reveals a Relationship between EGR3 Gene Modulation and Cell Migration Inhibition.Molecules (Basel, Switzerland) · 2024Article
- The Transcription Factor StuA Regulates the Glyoxylate Cycle in the DermatophyteInternational journal of molecular sciences · 2023Article
- Exploring the Therapeutic Potential ofInternational journal of molecular sciences · 2023Article
- Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis.Pharmaceutics · 2023Article
- The Transcriptional Regulation of Genes Involved in the Immune Innate Response of Keratinocytes Co-Cultured withJournal of fungi (Basel, Switzerland) · 2022Article
- Many ways, one microorganism: Several approaches to study Malassezia in interactions with model hosts.PLoS pathogens · 2022Review
- Anti-dermatophytic activity of cold atmospheric plasma against Trichophyton rubrum via affecting fungal growth, morphology, drug susceptibility and HSP90 gene expression.Scientific reports · 2022Article
- Isoflavaspidic Acid PB Extracted fromBioMed research international · 2022Article
- A first insight into the genome of Prototheca wickerhamii, a major causative agent of human protothecosis.BMC genomics · 2021Article
- Epidemiology and Diagnostic Perspectives of Dermatophytoses.Journal of fungi (Basel, Switzerland) · 2020Review
- Preparation, and Assessment of Antidermatophyte Activity of Miconazole-Urea Water-Soluble Film.Frontiers in microbiology · 2020Article
- HacA Governs Virulence Traits and Adaptive Stress Responses inFrontiers in microbiology · 2020Article
- The Transcriptional Profile ofPathogens (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Trichophyton rubrum is the most common causative agent of dermatomycoses worldwide, causing infection in the stratum corneum, nails, and hair. Despite the high prevalence of these infections, little is known about the molecular mechanisms involved in the fungal-host interaction, particularly during antifungal treatment. The aim of this work was to evaluate the gene expression of T. rubrum cocultured with keratinocytes and treated with the flavonoid trans-chalcone and the glycoalkaloid α-solanine. Both substances showed a marked antifungal activity against T. rubrum strain CBS (MIC = 1.15 and 17.8 µg/mL, resp.). Cytotoxicity assay against HaCaT cells produced IC50 values of 44.18 to trans-chalcone and 61.60 µM to α-solanine. The interaction of keratinocytes with T. rubrum conidia upregulated the expression of genes involved in the glyoxylate cycle, ergosterol synthesis, and genes encoding proteases but downregulated the ABC transporter TruMDR2 gene. However, both antifungals downregulated the ERG1 and ERG11, metalloprotease 4, serine proteinase, and TruMDR2 genes. Furthermore, the trans-chalcone downregulated the genes involved in the glyoxylate pathway, isocitrate lyase, and citrate synthase. Considering the urgent need for more efficient and safer antifungals, these results contribute to a better understanding of fungal-host interactions and to the discovery of new antifungal targets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.