ArticleFrontiers in microbiology2015
New multiplex PCR methods for rapid screening of genetically modified organisms in foods.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Screening method for detection of genetically modified soybean and maize events using multiplex PCR combined with capillary electrophoresis.GM crops & food · 2026Article
- Specific detection of genetically modified potatoes containingGM crops & food · 2025Article
- Tracing the botanical origins of UK heather honey by relative quantification of plant DNA.NPJ science of food · 2025Article
- Suitability of Real-Time PCR Methods for New Genomic Technique Detection in the Context of the European Regulations: A Case Study in Arabidopsis.International journal of molecular sciences · 2025Article
- A multi-channel CRISPR-based method for rapid, sensitive detection of four diseases ofHorticulture research · 2025Article
- Quantitative and qualitative analysis of three DNA extraction methods from soybean, maize, and canola oils and investigation of the presence of genetically modified organisms (GMOs).Food chemistry. Molecular sciences · 2024Article
- Multiplex PCR detection method of genetically modified canola event (MON94100, LBFLFK, and NS-B50027-4) combined with capillary electrophoresis.Food science and biotechnology · 2024Article
- Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences.Foods (Basel, Switzerland) · 2023Review
- Construction of transgenic detection system of3 Biotech · 2022Article
- Development of Multiplex PCR Coupled DNA Chip Technology for Assessment of Endogenous and Exogenous Allergens in GM Soybean.Biosensors · 2021Article
- Development of a Systematic qPCR Array for Screening GM Soybeans.Foods (Basel, Switzerland) · 2021Article
- A simple and accurate PCR method for detection of genetically modified rice.Journal of environmental health science & engineering · 2019Article
- Are Market GM Plants an Unrecognized Platform for Bioterrorism and Biocrime?Frontiers in bioengineering and biotechnology · 2019Article
- Threshold Level and Traceability of Roundup Ready® Soybeans in Tofu Production.Food technology and biotechnology · 2017Article
- Review
- Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize.Plant methods · 2017Article
- Rapid screening of roundup ready soybean in food samples by a hand-held PCR device.Food science and biotechnology · 2016Article
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5 authors.
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Abstract
We present novel multiplex PCR methods for rapid and reliable screening of genetically modified organisms (GMOs). New designed PCR primers targeting four frequently used GMO specific sequences permitted identification of new DNA markers, in particular 141 bp fragment of cauliflower mosaic virus (CaMV) 35S promoter, 224 bp fragment of Agrobacterium tumefaciens nopaline synthase (NOS) terminator, 256 bp fragment of 5-enolppyruvylshikimate-phosphate synthase (epsps) gene and 258 bp fragment of Cry1Ab delta-endotoxin (cry1Ab) gene for GMO screening. The certified reference materials containing Roundup Ready soybean (RRS) and maize MON 810 were applied for the development and optimization of uniplex and multiplex PCR systems. Evaluation of amplification products by agarose gel electrophoresis using negative and positive controls confirmed high specificity and sensitivity at 0.1% GMO for both RRS and MON 810. The fourplex PCR was developed and optimized that allows simultaneous detection of three common transgenic elements, such as: CaMV 35S promoter, NOS terminator, epsps gene together with soybean-specific lectin gene. The triplex PCR developed enables simultaneous identification of transgenic elements, such as: 35S promoter and cry1Ab gene together with maize zein gene. The analysis of different processed foods demonstrated that multiplex PCR methods developed in this study are useful for accurate and fast screening of GM food products.
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