ArticleFrontiers in physiology2015
Transcriptome analysis and RNA interference of cockroach phototransduction indicate three opsins and suggest a major role for TRPL channels.
Article in Frontiers in physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Comparative Neuroanatomy of Hydrothermal Vent Shrimps: Ecological Differentiation Among Four Alvinocaridid Species.The Journal of comparative neurology · 2026Article
- Baculoviruses hijack host midgut-derived tachykinin to regulate phototactic climbing behavior and promote viral transmission.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Improved chromosome-level genome assembly of the American cockroach, Periplaneta americana.G3 (Bethesda, Md.) · 2026Article
- Cephalic ganglia transcriptomics of the American cockroach Periplaneta americana (Blattodea: Blattidae).Journal of insect science (Online) · 2024Article
- Evolution of Opsin Genes in Caddisflies (Insecta: Trichoptera).Genome biology and evolution · 2024Article
- A Non-Gradual Development Process of Cicada Eyes at the End of the Fifth-Instar Nymphal Stage to Obtain Visual Ability.Insects · 2022Article
- Differential Gene Expression Correlates with Behavioural Polymorphism during Collective Behaviour in Cockroaches.Animals : an open access journal from MDPI · 2022Article
- Silencing the odorant receptor co-receptor impairs olfactory reception in a sensillum-specific manner in the cockroach.iScience · 2022Article
- The evolution of insect visual opsin genes with specific consideration of the influence of ocelli and life history traits.BMC ecology and evolution · 2022Article
- Five Visual and Olfactory Target Genes for RNAi inFrontiers in genetics · 2022Article
- Evidence for UV-green dichromacy in the basal hymenopteran Sirex noctilio (Siricidae).Scientific reports · 2021Article
- Molecular Evolution of Phototransduction Pathway Genes in Nocturnal and Diurnal Fireflies (Coleoptera: Lampyridae).Insects · 2021Article
- Gene transcription changes in a locust model of noise-induced deafness.Journal of neurophysiology · 2021Article
- Suppression of Gq and PLC gene expression has a small effect on quantum bumps in vivo in Periplaneta americana.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2020Article
- RNA interference supports a role for Nanchung-Inactive in mechanotransduction by the cockroach, Periplaneta americana, tactile spine.Invertebrate neuroscience : IN · 2020Article
- Evolution of Phototransduction Genes in Lepidoptera.Genome biology and evolution · 2019Article
- Identification and Characterization of theInternational journal of molecular sciences · 2019Article
- Effect of Capsaicin and Other Thermo-TRP Agonists on Thermoregulatory Processes in the American Cockroach.Molecules (Basel, Switzerland) · 2018Article
- Changes in electrophysiological properties of photoreceptors in Periplaneta americana associated with the loss of screening pigment.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2018Article
- Phenotypic plasticity inThe Journal of general physiology · 2018Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our current understanding of insect phototransduction is based on a small number of species, but insects occupy many different visual environments. We created the retinal transcriptome of a nocturnal insect, the cockroach, Periplaneta americana to identify proteins involved in the earliest stages of compound eye phototransduction, and test the hypothesis that different visual environments are reflected in different molecular contributions to function. We assembled five novel mRNAs: two green opsins, one UV opsin, and one each TRP and TRPL ion channel homologs. One green opsin mRNA (pGO1) was 100-1000 times more abundant than the other opsins (pGO2 and pUVO), while pTRPL mRNA was 10 times more abundant than pTRP, estimated by transcriptome analysis or quantitative PCR (qPCR). Electroretinograms were used to record photoreceptor responses. Gene-specific in vivo RNA interference (RNAi) was achieved by injecting long (596-708 bp) double-stranded RNA into head hemolymph, and verified by qPCR. RNAi of the most abundant green opsin reduced both green opsins by more than 97% without affecting UV opsin, and gave a maximal reduction of 75% in ERG amplitude 7 days after injection that persisted for at least 19 days. RNAi of pTRP and pTRPL genes each specifically reduced the corresponding mRNA by 90%. Electroretinogram (ERG) reduction by pTRPL RNAi was slower than for opsin, reaching 75% attenuation by 21 days, without recovery at 29 days. pTRP RNAi attenuated ERG much less; only 30% after 21 days. Combined pTRP plus pTRPL RNAi gave only weak evidence of any cooperative interactions. We conclude that silencing retinal genes by in vivo RNAi using long dsRNA is effective, that visible light transduction in Periplaneta is dominated by pGO1, and that pTRPL plays a major role in cockroach phototransduction.
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