ArticleProtein science : a publication of the Protein Society2015
The structure of the Caenorhabditis elegans manganese superoxide dismutase MnSOD-3-azide complex.
Article in Protein science : a publication of the Protein Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- The curious life of human mitochondrial SOD2.International journal of biological macromolecules · 2026Review
- The structure-function relationships and physiological roles of MnSOD mutants.Bioscience reports · 2022Review
- In-gel SOD assay reveals SOD-2 is the single active, water-soluble SOD enzyme inFree radical research · 2021Article
- Substitution of histidine 30 by asparagine in manganese superoxide dismutase alters biophysical properties and supports proliferation in a K562 leukemia cell line.European biophysics journal : EBJ · 2021Article
- Optimization in continuous phase-transition extraction of crude flavonoids from finger citron fruit and evaluation on their antiaging activities.Food science & nutrition · 2020Article
- Article
- Redox manipulation of the manganese metal in human manganese superoxide dismutase for neutron diffraction.Acta crystallographica. Section F, Structural biology communications · 2018Article
- A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
- The first crystal structure of manganese superoxide dismutase from the genus Staphylococcus.Acta crystallographica. Section F, Structural biology communications · 2018Article
- A Review of the Catalytic Mechanism of Human Manganese Superoxide Dismutase.Antioxidants (Basel, Switzerland) · 2018Review
- Substrate-analog binding and electrostatic surfaces of human manganese superoxide dismutase.Journal of structural biology · 2017Article
- The Superoxide Dismutase Mimetic M40403, Improves 5-Fluorouracil-induced Small Intestinal Mucositis in a Mouse Model.In vivo (Athens, Greece)Article
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6 authors.
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Abstract
C. elegans MnSOD-3 has been implicated in the longevity pathway and its mechanism of catalysis is relevant to the aging process and carcinogenesis. The structures of MnSOD-3 provide unique crystallographic evidence of a dynamic region of the tetrameric interface (residues 41-54). We have determined the structure of the MnSOD-3-azide complex to 1.77-Å resolution. Analysis of this complex shows that the substrate analog, azide, binds end-on to the manganese center as a sixth ligand and that it ligates directly to a third and new solvent molecule also positioned within interacting distance to the His30 and Tyr34 residues of the substrate access funnel. This is the first structure of a eukaryotic MnSOD-azide complex that demonstrates the extended, uninterrupted hydrogen-bonded network that forms a proton relay incorporating three outer sphere solvent molecules, the substrate analog, the gateway residues, Gln142, and the solvent ligand. This configuration supports the formation and release of the hydrogen peroxide product in agreement with the 5-6-5 catalytic mechanism for MnSOD. The high product dissociation constant k4 of MnSOD-3 reflects low product inhibition making this enzyme efficient even at high levels of superoxide.
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