Evidence map›Paper›PMID 26257319›Full record

ArticleMacromolecular bioscience2016

Efficient pDNA Delivery Using Cationic 2-Hydroxypropyl-β-Cyclodextrin Pluronic-Based Polyrotaxanes.

Vivek Badwaik, Yawo Mondjinou, Aditya Kulkarni, Linjia Liu, Asher Demoret, David H Thompson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Macromolecular bioscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. One-pot synthesis of block-copolyrotaxanes through controlledBeilstein journal of organic chemistry · 2017
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Vivek BadwaikDepartment of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
Yawo MondjinouDepartment of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
Aditya KulkarniDepartment of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
Linjia LiuDepartment of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
Asher DemoretDepartment of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA.
David H ThompsonDepartment of Chemistry, Multi-Disciplinary Cancer Research Facility, Bindley Bioscience Center, 1203 W. State Street, West Lafayette, Indiana 47907, USA. davethom@purdue.edu.

Funding

Transgenic Mouse Core Facility Shared Resource (TMCF-SR)P30CA023168 · NCI · PURDUE UNIVERSITY WEST LAFAYETTE · PI John Tesmer · 1985 to 2026
$43.4M
Development of Bioresponsive Lipids for Intracellular DeliveryR01GM087016 · NIGMS · PURDUE UNIVERSITY · PI THOMPSON, DAVID H · 2009 to 2012
$1.2M
Development of Long-circulating, Degradable Gd-Polyrotaxane MR AgentsR21EB017921 · NIBIB · PURDUE UNIVERSITY · PI THOMPSON, DAVID H · 2014 to 2015
$432k
Targeting of Bladder Tumor Cells by Nanocarriers Bearing bacillus Calmette-GuerinR21CA151961 · NCI · PURDUE UNIVERSITY · PI AGUILAR, RUBEN CLAUDIO · 2013 to 2014
$352k
NCI NIH HHS P30 CA023168NCI NIH HHS R21 CA15196NCI NIH HHS R21 CA151961NIBIB NIH HHS R21 EB017921NIGMS NIH HHS NIH RO1 GM087016NIGMS NIH HHS R01 GM087016
6 · The paper itself

Abstract

A family of cationic Pluronic-based polyrotaxanes (PR(+)), threaded with 2-hydroxypropyl-β-cyclodextrin (HPCD), was synthesized for pDNA delivery into multiple cell lines. All PR(+) formed highly stable, positively charged pDNA complexes that were < 250 nm in diameter. The cellular uptake and pDNA transfection efficiencies of the PR(+):pDNA complexes was enhanced relative to the commercial transfection standards L2K and bPEI, while displaying similar or lower toxicity profiles. Charge density and threading efficiency of the PR(+) agent significantly influenced the colloidal stability and physical properties of the complexes, which impacted their intracellular transfection efficiencies. Taken together, our results suggest that HPCD: Pluronic PR(+) can be used as potent vectors for pDNA-based therapeutics.

Indexed as

Plasmids2-Hydroxypropyl-beta-cyclodextrinbeta-CyclodextrinsCell LineHumansPoloxamerRotaxanesTransfection2-Hydroxypropyl-beta-cyclodextrinbeta-CyclodextrinsPoloxamerRotaxanesgene deliverypDNApolyrotaxanetransfectionβ-cyclodextrins

Identifiers

PMID26257319
PMCPMC4891183

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.