ArticleAndrology2015
A detailed protocol for a rapid analysis of testicular cell populations using flow cytometry.
Article in Andrology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Environmentally relevant DEHP exposure during gestational and lactational period inhibits filamin a testicular expression.Journal of molecular histology · 2023Article
- Generation of rat offspring using spermatids produced through in vitro spermatogenesis.Scientific reports · 2023Article
- Human-specific gene CT47 blocks PRMT5 degradation to lead to meiosis arrest.Cell death discovery · 2022Article
- Long-term effects of early postnatal stress on Sertoli cells.Frontiers in genetics · 2022Article
- Disruption of anchoring junctions in the testes of experimental varicocele rats.Experimental and therapeutic medicine · 2021Article
- Cellular Expression and Subcellular Localization of Wwox Protein During Testicular Development and Spermatogenesis in Rats.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2021Article
- Loss of Profilin3 Impairs Spermiogenesis by Affecting Acrosome Biogenesis, Autophagy, Manchette Development and Mitochondrial Organization.Frontiers in cell and developmental biology · 2021Article
- S100A4+ macrophages facilitate zika virus invasion and persistence in the seminiferous tubules via interferon-gamma mediation.PLoS pathogens · 2020Article
- Characterization of rodent Sertoli cell primary cultures.Molecular reproduction and development · 2020Review
- The intramembrane protease SPPL2c promotes male germ cell development by cleaving phospholamban.EMBO reports · 2019Article
- Genotypic and Phenotypic Variables Affect Meiotic Cell Cycle Progression, Tumor Ploidy, and Cancer-Associated Mortality in aJournal of oncology · 2019Article
- Single-cell RNA sequencing of adult mouse testes.Scientific data · 2018Article
- Characterization of germ cell differentiation in the male mouse through single-cell RNA sequencing.Scientific reports · 2018Article
- Fertility preservation issues in pediatric hematopoietic stem cell transplantation: practical approaches from the consensus of the Pediatric Diseases Working Party of the EBMT and the International BFM Study Group.Bone marrow transplantation · 2017Article
- Knock-Out Serum Replacement and Melatonin Effects on Germ Cell Differentiation in Murine Testicular Explant Cultures.Annals of biomedical engineering · 2017Article
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Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate analysis and quantification of different testicular cell populations are of central importance in studies of male reproductive biology. The traditional histomorphometric and immunohistochemical methods remain the gold standard in studying the complex dynamics of the testicular tissue. Through past years advances have been made in the application of flow cytometry for the rapid analysis of testicular cell populations. Detection of DNA content and of surface antigens and fluorescent reporters have been widely used to analyze and sort cells. Detection of intracellular antigens can broaden the possibilities of applying flow cytometry in studies of male reproduction. Here, we report a detailed protocol for the preparation of rat testicular tissue for detection of intracellular antigens by flow cytometry, and a pipeline for subsequent data analysis and troubleshooting. Rat testicular ontogenesis was chosen as the experimental model to validate the performance of the assay using vimentin and γH2AX as intracellular markers for the somatic and spermatogenic cells, respectively. The results show that the assay is reproducible and recapitulates the rat testis ontogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.