ArticleCurrent biology : CB2015
DNA Replication Initiation Is Blocked by a Distant Chromosome-Membrane Attachment.
Article in Current biology : CB, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Construction of synthetic protein-binding non-genetic DNA systems in living cells.Nature chemistry · 2026Article
- Spatio-temporal organization of theEcoSal Plus · 2024Review
- Article
- Psoralen mapping reveals a bacterial genome supercoiling landscape dominated by transcription.Nucleic acids research · 2022Article
- XerD unloads bacterial SMC complexes at the replication terminus.Molecular cell · 2021Article
- Nuclear filaments: role in chromosomal positioning and gene expression.Nucleus (Austin, Tex.) · 2020Review
- Proteome Mapping of a Cyanobacterium Reveals Distinct Compartment Organization and Cell-Dispersed Metabolism.Plant physiology · 2019Article
- Expression of different ParE toxins results in conserved phenotypes with distinguishable classes of toxicity.MicrobiologyOpen · 2019Article
- The Stringent Response Inhibits DNA Replication Initiation in E. coli by Modulating Supercoiling ofmBio · 2019Article
- DNA gyrase activity regulates DnaA-dependent replication initiation in Bacillus subtilis.Molecular microbiology · 2018Article
- Coordination of Growth, Chromosome Replication/Segregation, and Cell Division inFrontiers in microbiology · 2018Article
- Review
- Multilocus Imaging of the E. coli Chromosome by Fluorescent In Situ Hybridization.Methods in molecular biology (Clifton, N.J.) · 2017Article
- CRISPR-mediated control of the bacterial initiation of replication.Nucleic acids research · 2016Article
- Regulation of DNA Replication Initiation by Chromosome Structure.Journal of bacteriology · 2015Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
Abstract
Although it has been recognized for several decades that chromosome structure regulates the capacity of replication origins to initiate, very little is known about how or if cells actively regulate structure to direct initiation. We report that a localized inducible protein tether between the chromosome and cell membrane in E. coli cells imparts a rapid and complete block to replication initiation. Tethers, composed of a trans-membrane and transcription repressor fusion protein bound to an array of operator sequences, can be placed up to 1 Mb from the origin with no loss of penetrance. Tether-induced initiation blocking has no effect on elongation at pre-existing replication forks and does not cause cell or DNA damage. Whole-genome and site-specific fluorescent DNA labeling in tethered cells indicates that global nucleoid structure and chromosome organization are disrupted. Gene expression patterns, assayed by RNA sequencing, show that tethering induces global supercoiling changes, which are likely incompatible with replication initiation. Parallels between tether-induced initiation blocking and rifampicin treatment and the role of programmed changes in chromosome structure in replication control are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.