ArticleJournal of molecular biology2015
Influence of Internal DNA Pressure on Stability and Infectivity of Phage λ.
Article in Journal of molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Stabilization mechanism accommodating genome length variation in evolutionarily related viral capsids.Nature communications · 2025Article
- Bacteriophage Challenges in Industrial Processes: A Historical Unveiling and Future Outlook.Pathogens (Basel, Switzerland) · 2024Review
- An Overview of Diverse Strategies To InactivateEcoSal Plus · 2023Review
- Geochemical constraints on bacteriophage infectivity in terrestrial environments.ISME communications · 2023Article
- Selection and characterization of bacteriophages specific toVeterinary world · 2022Article
- Thermoresponsive C22 phage stiffness modulates the phage infectivity.Scientific reports · 2022Article
- Heat adaptation of phage T7 under an extended genetic code.Virus evolution · 2021Article
- The PLB measurement for the connector in Phi29 bacteriophage reveals the function of its channel loop.Biophysical journal · 2021Article
- Principles for enhancing virus capsid capacity and stability from a thermophilic virus capsid structure.Nature communications · 2019Article
- Article
- Influence of Microscopic Interactions on the Flexible Mechanical Properties of Viral DNA.Biophysical journal · 2018Article
- A Hyperthermophilic Phage Decoration Protein Suggests Common Evolutionary Origin with Herpesvirus Triplex Proteins and an Anti-CRISPR Protein.Structure (London, England : 1993) · 2018Article
- Virus and Host Mechanics Support Membrane Penetration and Cell Entry.Journal of virology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Viruses must remain infectious while in harsh extracellular environments. An important aspect of viral particle stability for double-stranded DNA viruses is the energetically unfavorable state of the tightly confined DNA chain within the virus capsid creating pressures of tens of atmospheres. Here, we study the influence of internal genome pressure on the thermal stability of viral particles. Using differential scanning calorimetry to monitor genome loss upon heating, we find that internal pressure destabilizes the virion, resulting in a smaller activation energy barrier to trigger DNA release. These experiments are complemented by plaque assay and electron microscopy measurements to determine the influence of intra-capsid DNA pressure on the rates of viral infectivity loss. At higher temperatures (65-75°C), failure to retain the packaged genome is the dominant mechanism of viral inactivation. Conversely, at lower temperatures (40-55°C), a separate inactivation mechanism dominates, which results in non-infectious particles that still retain their packaged DNA. Most significantly, both mechanisms of infectivity loss are directly influenced by internal DNA pressure, with higher pressure resulting in a more rapid rate of inactivation at all temperatures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.