ArticleAmerican journal of physiology. Lung cellular and molecular physiology2015
In vivo compartmental analysis of leukocytes in mouse lungs.
Article in American journal of physiology. Lung cellular and molecular physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Multiomics immune profiling of a patient-relevant orthotopic lung cancer model using SEPARATE-Seq.Nature communications · 2026Article
- Intravascular immune cell labeling as a critical methodological tool for characterizing CD4Frontiers in immunology · 2026Article
- Multicompartmental analysis of the murine pulmonary immune response by spectral flow cytometry.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Deciphering decidual leukocyte traffic with serial intravascular staining.Frontiers in immunology · 2023Article
- Locally organised and activated Fth1Nature communications · 2022Article
- Lysophosphatidylcholine Alleviates Acute Lung Injury by Regulating Neutrophil Motility and Neutrophil Extracellular Trap Formation.Frontiers in cell and developmental biology · 2022Article
- Regulatory mechanisms of neutrophil migration from the circulation to the airspace.Cellular and molecular life sciences : CMLS · 2021Review
- CD49a Identifies Polyfunctional Memory CD8 T Cell Subsets that Persist in the Lungs After Influenza Infection.Frontiers in immunology · 2021Article
- Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS.Nature protocols · 2020Article
- Distinctive Cellular and Metabolic Reprogramming in Porcine Lung Mononuclear Phagocytes Infected With Type 1 PRRSV Strains.Frontiers in immunology · 2020Article
- Monocytes mediate homing of circulating microvesicles to the pulmonary vasculature during low-grade systemic inflammation.Journal of extracellular vesicles · 2020Article
- Redistribution of EC-SOD resolves bleomycin-induced inflammationFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Ventilation following established ARDS: a preclinical model framework to improve predictive power.Thorax · 2019Article
- RAGE is a Critical Mediator of Pulmonary Oxidative Stress, Alveolar Macrophage Activation and Emphysema in Response to Cigarette Smoke.Scientific reports · 2019Article
- In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Porcine Alveolar Macrophage-like cells are pro-inflammatory Pulmonary Intravascular Macrophages that produce large titers of Porcine Reproductive and Respiratory Syndrome Virus.Scientific reports · 2018Article
- Intravascular donor monocytes play a central role in lung transplant ischaemia-reperfusion injury.Thorax · 2018Article
- Article
- Phagocytosis of microparticles by alveolar macrophages during acute lung injury requires MerTK.American journal of physiology. Lung cellular and molecular physiology · 2018Article
- Deletion of c-FLIP from CD11bAmerican journal of respiratory cell and molecular biology · 2018Article
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Authors and funding
5 authors.
Funding
Abstract
The lung has a unique structure consisting of three functionally different compartments (alveolar, interstitial, and vascular) situated in an extreme proximity. Current methods to localize lung leukocytes using bronchoalveolar lavage and/or lung perfusion have significant limitations for determination of location and phenotype of leukocytes. Here we present a novel method using in vivo antibody labeling to enable accurate compartmental localization/quantification and phenotyping of mouse lung leukocytes. Anesthetized C57BL/6 mice received combined in vivo intravenous and intratracheal labeling with fluorophore-conjugated anti-CD45 antibodies, and lung single-cell suspensions were analyzed by flow cytometry. The combined in vivo intravenous and intratracheal CD45 labeling enabled robust separation of the alveolar, interstitial, and vascular compartments of the lung. In naive mice, the alveolar compartment consisted predominantly of resident alveolar macrophages. The interstitial compartment, gated by events negative for both intratracheal and intravenous CD45 staining, showed two conventional dendritic cell populations, as well as a Ly6C(lo) monocyte population. Expression levels of MHCII on these interstitial monocytes were much higher than on the vascular Ly6C(lo) monocyte populations. In mice exposed to acid aspiration-induced lung injury, this protocol also clearly distinguished the three lung compartments showing the dynamic trafficking of neutrophils and exudative monocytes across the lung compartments during inflammation and resolution. This simple in vivo dual-labeling technique substantially increases the accuracy and depth of lung flow cytometric analysis, facilitates a more comprehensive examination of lung leukocyte pools, and enables the investigation of previously poorly defined "interstitial" leukocyte populations during models of inflammatory lung diseases.
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