ArticleMethods in enzymology2015
Pseudouridine in mRNA: Incorporation, Detection, and Recoding.
Article in Methods in enzymology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 16 citations in OpenAlex.
- Nanopore signal deviations from pseudouridine modifications in RNA are sequence-specific: quantification requires dedicated synthetic controls.Scientific reports · 2024Article
- mRNA-based HIV-1 vaccines.Clinical microbiology reviews · 2024Review
- Epigenetic and gene therapy in human and veterinary medicine.Environmental epigenetics · 2024Review
- Review
- Pseudouridine as a novel biomarker in prostate cancer.Urologic oncology · 2021Review
- The Arabidopsis 2'-O-Ribose-Methylation and Pseudouridylation Landscape of rRNA in Comparison to Human and Yeast.Frontiers in plant science · 2021Review
- Regulation and Function of RNA Pseudouridylation in Human Cells.Annual review of genetics · 2020Review
- A Census and Categorization Method of Epitranscriptomic Marks.International journal of molecular sciences · 2020Review
- Pre-mRNA modifications and their role in nuclear processing.Quantitative biology (Beijing, China) · 2018Article
- Guide-substrate base-pairing requirement for box H/ACA RNA-guided RNA pseudouridylation.RNA (New York, N.Y.) · 2018Article
- Going global: the new era of mapping modifications in RNA.Wiley interdisciplinary reviews. RNA · 2017Review
- Evolving insights into RNA modifications and their functional diversity in the brain.Nature neuroscience · 2016Article
- Structural Basis for Translation Termination on a Pseudouridylated Stop Codon.Journal of molecular biology · 2016Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
It has long been known that pseudouridine (Ψ) is the most abundant modified nucleotide in stable RNAs, including tRNA, rRNA, and snRNA. Recent studies using massive parallel sequencing have uncovered the presence of hundreds of Ψs in mRNAs as well. In eukaryotes and archaea, RNA pseudouridylation is introduced predominantly by box H/ACA RNPs, RNA-protein complexes each consisting of a single RNA moiety and four core proteins. It has been well established that Ψ plays an essential role in regulating the structure and function of stable RNAs in several model organisms, including yeast, Xenopus laevis, and humans. However, the functional role of Ψ in mRNA remains to be elucidated. One possibility (and true for stop/termination codons) is that Ψ influences decoding during translation. It is imperative, therefore, to establish a system, in which one can site-specifically introduce pseudouridylation into target mRNA and biochemically test the impact of mRNA pseudouridylation on protein translation. Here, we present a method for (1) site-specific conversion of uridine into Ψ in mRNA by designer box H/ACA RNP, (2) detection of Ψ in target mRNA using site-specific labeling followed by nuclease digestion and thin layer chromatography, and (3) analysis of recoding of pseudouridylated premature termination codon in mRNA during translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.