ArticleBMC plant biology2015
Transcriptional transitions in Nicotiana benthamiana leaves upon induction of oil synthesis by WRINKLED1 homologs from diverse species and tissues.
Article in BMC plant biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 1 of them a synthesis that pooled it.
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Who cites it
71 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Advancements in Crop PUFAs Biosynthesis and Genetic Engineering: A Systematic and Mixed Review System.International journal of molecular sciences · 2025Pooled it
- From wrinkled seeds to plant oil accumulation networks: the legacy of a Plant Physiology classic.Plant physiology · 2026Review
- Oil as a Hindrance to Oat (Foods (Basel, Switzerland) · 2026Review
- Identification ofPlant biotechnology (Tokyo, Japan) · 2025Article
- The transcription factor CeWRI3 functions in TAG accumulation through activating CeOLE2 in Cyperus esculentus.Plant cell reports · 2025Article
- Effects of DpWRI1-like gene overexpression in transgenic Dunaliella parva.Antonie van Leeuwenhoek · 2025Article
- SIZ1 SUMOylates and stabilizes WRI1 to safeguard seed filling and fatty acid biosynthesis under high-temperature stress.The Plant cell · 2025Article
- Arachis hypogaea monoacylglycerol lipase AhMAGL3b participates in lipid metabolism.BMC plant biology · 2024Article
- Article
- Spatio-temporal transcriptome and storage compound profiles of developing faba bean (Frontiers in plant science · 2024Article
- Lipid droplets inFrontiers in plant science · 2024Article
- Molecular characterization of oleosin genes in Cyperus esculentus, a Cyperaceae plant producing oil in underground tubers.Plant cell reports · 2023Article
- A Study on the Use of the Phyto-Courier Technology in Tobacco Leaves Infected byInternational journal of molecular sciences · 2023Article
- Improved bioenergy value of residual rice straw by increased lipid levels from upregulation of fatty acid biosynthesis.Biotechnology for biofuels and bioproducts · 2023Article
- Ectopic Expression ofPlants (Basel, Switzerland) · 2023Article
- Engineering triacylglycerol accumulation in duckweed (Lemna japonica).Plant biotechnology journal · 2023Article
- Transcriptional regulation of oil biosynthesis in seed plants: Current understanding, applications, and perspectives.Plant communications · 2022Review
- Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis.Science advances · 2022Article
- Expression of AtWRI1 and AtDGAT1 during soybean embryo development influences oil and carbohydrate metabolism.Plant biotechnology journal · 2022Article
- Pathway Engineering, Re-targeting, and Synthetic Scaffolding Improve the Production of Squalene in Plants.ACS synthetic biology · 2022Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCarbon accumulation and remobilization are essential mechanisms in plants to ensure energy transfer between plant tissues with different functions or metabolic needs and to support new generations. Knowledge about the regulation of carbon allocation into oil (triacylglycerol) in plant storage tissue can be of great economic and environmental importance for developing new high-yielding oil crops. Here, the effect on global gene expression as well as on physiological changes in leaves transiently expressing five homologs of the transcription factor WRINKLED1 (WRI1) originating from diverse species and tissues; Arabidopsis thaliana and potato (Solanum tuberosum) seed embryo, poplar (Populus trichocarpa) stem cambium, oat (Avena sativa) grain endosperm, and nutsedge (Cyperus esculentus) tuber parenchyma, were studied by agroinfiltration in Nicotiana benthamiana.
resultsAll WRI1 homologs induced oil accumulation when expressed in leaf tissue. Transcriptome sequencing revealed that all homologs induced the same general patterns with a drastic shift in gene expression profiles of leaves from that of a typical source tissue to a source-limited sink-like tissue: Transcripts encoding enzymes for plastid uptake and metabolism of phosphoenolpyruvate, fatty acid and oil biosynthesis were up-regulated, as were also transcripts encoding starch degradation. Transcripts encoding enzymes in photosynthesis and starch synthesis were instead down-regulated. Moreover, transcripts representing fatty acid degradation were up-regulated indicating that fatty acids might be degraded to feed the increased need to channel carbons into fatty acid synthesis creating a futile cycle. RT-qPCR analysis of leaves expressing Arabidopsis WRI1 showed the temporal trends of transcripts selected as 'markers' for key metabolic pathways one to five days after agroinfiltration. Chlorophyll fluorescence measurements of leaves expressing Arabidopsis WRI1 showed a significant decrease in photosynthesis, even though effect on starch content could not be observed.
conclusionsThis data gives for the first time a general view on the transcriptional transitions in leaf tissue upon induction of oil synthesis by WRI1. This yields important information about what effects WRI1 may exert on global gene expression during seed and embryo development. The results suggest why high oil content in leaf tissue cannot be achieved by solely transcriptional activation by WRI1, which can be essential knowledge in the development of new high-yielding oil crops.
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