ArticleApplied and environmental microbiology2015
phoD Alkaline Phosphatase Gene Diversity in Soil.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
75 citing papers in PubMed, 336 citations in OpenAlex.
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- Impact of Dominant Species Shift in Herbaceous Vegetation Beneath Sand-Fixing Plantations on Soil Microbial Communities Involved in Organic P Mineralization and Inorganic P Solubilization.Plants (Basel, Switzerland) · 2026Article
- Microbial phosphorus cycling in terrestrial ecosystems.Nature reviews. Microbiology · 2026Review
- Stage-Dependent Dynamics and Assembly Processes ofMicroorganisms · 2026Article
- Enhancing Phosphorus Availability Through Bagasse Biochar Addition and Changes inMicroorganisms · 2026Article
- Plant-Derived Organic Acids Are Linked to Arbuscular Mycorrhizal Fungi andMicroorganisms · 2026Article
- Unraveling plasmid contributions to phosphorus acquisition in soil microbiomes.Environmental microbiome · 2026Article
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- Compartment-Specific Niche Filtering Shapes the Structure and Nutrient-Cycling Potential of Bacterial Communities in Eutrophic Waters withPlants (Basel, Switzerland) · 2026Article
- Nutrient limitation shapes functional traits of mycorrhizal fungi and phosphorus-cycling bacteria across an elevation gradient.mSystems · 2026Article
- Nano-zeolite-coupled biochar-based phosphorus fertilizer enhances soil phosphorus availability and leaf phosphorus concentration in Moso bamboo forests.Frontiers in plant science · 2026Article
- The distribution and kinetics of phosphatase activity in European agricultural soils under long-term tillage reduction practices.Sustainable microbiology · 2026Article
- Editorial: Microbial-driven carbon, nitrogen and phosphorus cycling mechanisms in terrestrial ecosystems.Frontiers in microbiology · 2026Article
- Organic-mineral fertilization modulates microbial communities and nutrient-cycling genes in saline-alkali soil.Frontiers in microbiology · 2026Article
- Chloroflexota in agricultural soils: current knowledge and future research directions.Frontiers in microbiology · 2026Review
- Signatures of local nitrogen adaptation in the Brachypodium distachyon root microbiome.The New phytologist · 2026Article
- Manure substitution counteracts mineral nitrogen-driven erosion of theISME communications · 2026Article
15 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatase enzymes are responsible for much of the recycling of organic phosphorus in soils. The PhoD alkaline phosphatase takes part in this process by hydrolyzing a range of organic phosphoesters. We analyzed the taxonomic and environmental distribution of phoD genes using whole-genome and metagenome databases. phoD alkaline phosphatase was found to be spread across 20 bacterial phyla and was ubiquitous in the environment, with the greatest abundance in soil. To study the great diversity of phoD, we developed a new set of primers which targets phoD genes in soil. The primer set was validated by 454 sequencing of six soils collected from two continents with different climates and soil properties and was compared to previously published primers. Up to 685 different phoD operational taxonomic units were found in each soil, which was 7 times higher than with previously published primers. The new primers amplified sequences belonging to 13 phyla, including 71 families. The most prevalent phoD genes identified in these soils were affiliated with the orders Actinomycetales (13 to 35%), Bacillales (1 to 29%), Gloeobacterales (1 to 18%), Rhizobiales (18 to 27%), and Pseudomonadales (0 to 22%). The primers also amplified phoD genes from additional orders, including Burkholderiales, Caulobacterales, Deinococcales, Planctomycetales, and Xanthomonadales, which represented the major differences in phoD composition between samples, highlighting the singularity of each community. Additionally, the phoD bacterial community structure was strongly related to soil pH, which varied between 4.2 and 6.8. These primers reveal the diversity of phoD in soil and represent a valuable tool for the study of phoD alkaline phosphatase in environmental samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.