ArticleApplied and environmental microbiology2015
Gene deletion strategy to examine the involvement of the two chondroitin lyases in Flavobacterium columnare virulence.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Opportunistic diseases in marine eukaryotes: Could Bacteroidota be the next threat to ocean life?Environmental microbiology · 2022Pooled it
- Pleiotropic effects of signal peptide peptidase A (Frontiers in veterinary science · 2026Article
- Gliding motility proteins GldJ and SprB contribute toJournal of bacteriology · 2024Article
- The secretome of the fish pathogenFrontiers in cellular and infection microbiology · 2023Article
- Secreted peptidases contribute to virulence of fish pathogenFrontiers in cellular and infection microbiology · 2023Article
- Siderophores Produced by the Fish Pathogen Flavobacterium columnare Strain MS-FC-4 Are Not Essential for Its Virulence.Applied and environmental microbiology · 2022Article
- Riemerella anatipestifer T9SS Effector SspA Functions in Bacterial Virulence and Defending Natural Host Immunity.Applied and environmental microbiology · 2022Article
- Type IX Secretion System Effectors and Virulence of the Model Flavobacterium columnare Strain MS-FC-4.Applied and environmental microbiology · 2022Article
- Article
- Light stimulates anoxic and oligotrophic growth of glacial Flavobacterium strains that produce zeaxanthin.The ISME journal · 2021Article
- Cooperation between Different CRISPR-Cas Types Enables Adaptation in an RNA-Targeting System.mBio · 2021Article
- Prevailing Role of Mucosal Igs and B Cells in Teleost Skin Immune Responses to Bacterial Infection.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Advancements in CharacterizingPathogens (Basel, Switzerland) · 2020Review
- The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium psychrophilum.Applied and environmental microbiology · 2020Article
- Article
- The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium columnare.Applied and environmental microbiology · 2017Article
- The Complete Genome Sequence of the Fish PathogenFrontiers in microbiology · 2017Article
- High Nutrient Concentration Can Induce Virulence Factor Expression and Cause Higher Virulence in an Environmentally Transmitted Pathogen.Microbial ecology · 2016Article
- Intensive aquaculture selects for increased virulence and interference competition in bacteria.Proceedings. Biological sciences · 2016Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavobacterium columnare is an important bacterial pathogen of freshwater fish that causes high mortality of infected fish and heavy economic losses in aquaculture. The pathogenesis of this bacterium is poorly understood, in part due to the lack of efficient methods for genetic manipulation. In this study, a gene deletion strategy was developed and used to determine the relationship between the production of chondroitin lyases and virulence. The F. johnsoniae ompA promoter (PompA) was fused to sacB to construct a counterselectable marker for F. columnare. F. columnare carrying PompA-sacB failed to grow on media containing 10% sucrose. A suicide vector carrying PompA-sacB was constructed, and a gene deletion strategy was developed. Using this approach, the chondroitin lyase-encoding genes, cslA and cslB, were deleted. The ΔcslA and ΔcslB mutants were both partially deficient in digestion of chondroitin sulfate A, whereas a double mutant (ΔcslA ΔcslB) was completely deficient in chondroitin lyase activity. Cells of F. columnare wild-type strain G4 and of the chondroitin lyase-deficient ΔcslA ΔcslB mutant exhibited similar levels of virulence toward grass carp in single-strain infections. Coinfections, however, revealed a competitive advantage for the wild type over the chondroitin lyase mutant. The results indicate that chondroitin lyases are not essential virulence factors of F. columnare but may contribute to the ability of the pathogen to compete and cause disease in natural infections. The gene deletion method developed in this study may be employed to investigate the virulence factors of this bacterium and may have wide application in many other members of the phylum Bacteroidetes.
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