Evidence map›Paper›PMID 26253512›Full record

ArticleRetrovirology2015

Human and murine APOBEC3s restrict replication of koala retrovirus by different mechanisms.

Takayuki Nitta, Dat Ha, Felipe Galvez, Takayuki Miyazawa, Hung Fan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Retrovirology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Molecular Diagnosis of Koala Retrovirus (KoRV) in South Australian Koalas (Animals : an open access journal from MDPI · 2021
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Takayuki NittaDepartment of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697-3905, USA. nittat@savannahstate.edu.
Dat HaDepartment of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697-3905, USA. datha@usc.edu.
Felipe GalvezDepartment of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697-3905, USA. fgalvezp6@gmail.com.
Takayuki MiyazawaLaboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, 606-8507, Japan. takavet@gmail.com.
Hung FanDepartment of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697-3905, USA. hyfan@uci.edu.
University of California, Irvine · USKyoto University · JP

Funding

Oncogenesis By Jaagsiekte Sheep RetrovirusR01CA094188 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI FAN, HUNG Y. · 2002 to 2011
$3.2M
NCI NIH HHS CA94188NCI NIH HHS R01 CA094188
6 · The paper itself

Abstract

backgroundKoala retrovirus (KoRV) is an endogenous and exogenous retrovirus of koalas that may cause lymphoma. As for many other gammaretroviruses, the KoRV genome can potentially encode an alternate form of Gag protein, glyco-gag.

resultsIn this study, a convenient assay for assessing KoRV infectivity in vitro was employed: the use of DERSE cells (initially developed to search for infectious xenotropic murine leukemia-like viruses). Using infection of DERSE and other human cell lines (HEK293T), no evidence for expression of glyco-gag by KoRV was found, either in expression of glyco-gag protein or changes in infectivity when the putative glyco-gag reading frame was mutated. Since glyco-gag mediates resistance of Moloney murine leukemia virus to the restriction factor APOBEC3, the sensitivity of KoRV (wt or putatively mutant for glyco-gag) to restriction by murine (mA3) or human APOBEC3s was investigated. Both mA3 and hA3G potently inhibited KoRV infectivity. Interestingly, hA3G restriction was accompanied by extensive G → A hypermutation during reverse transcription while mA3 restriction was not. Glyco-gag status did not affect the results.

conclusionsThese results indicate that the mechanisms of APOBEC3 restriction of KoRV by hA3G and mA3 differ (deamination dependent vs. independent) and glyco-gag does not play a role in the restriction.

Indexed as

Virus ReplicationAmino Acid SequenceAnimalsAPOBEC DeaminasesCytidine DeaminaseCytosine DeaminaseGammaretrovirusGene Products, gagHEK293 CellsHumansMiceOpen Reading FramesPhascolarctidaeReverse TranscriptionSequence AlignmentApobec3 protein, mouseAPOBEC3 proteins, humanAPOBEC DeaminasesCytidine DeaminaseCytosine DeaminaseGene Products, gag

Identifiers

PMID26253512
PMCPMC4528783
OpenAlexW1902123650

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.