ArticleDevelopment (Cambridge, England)2015
Kinesin-1 interacts with Bucky ball to form germ cells and is required to pattern the zebrafish body axis.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Maternal Huluwa regulates postfertilization microtubule array organization for asymmetrical transport of dorsal determinants in zebrafish.Nature communications · 2026Article
- Primordial Germ Cell Development in Teleosts: Their Origin, Fate and Role in Sex Determination.Stem cell reviews and reports · 2026Review
- Article
- Dynamic maternal synthesis and segregation of the germ plasm organizer, Bucky ball, in chicken oocytes and follicles.Scientific reports · 2024Article
- Yolk granule fusion and microtubule aster formation regulate cortical granule translocation and exocytosis in zebrafish oocytes.PLoS biology · 2023Article
- The midbody component Prc1-like is required for microtubule reorganization during cytokinesis and dorsal determinant segregation in the early zebrafish embryo.Development (Cambridge, England) · 2023Article
- Sinhcaf-dependent histone deacetylation is essential for primordial germ cell specification.EMBO reports · 2022Article
- Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants.Journal of visualized experiments : JoVE · 2021Article
- A transgenic system for targeted ablation of reproductive and maternal-effect genes.Development (Cambridge, England) · 2021Article
- The developmental biology of kinesins.Developmental biology · 2021Review
- Molecular asymmetry in the cephalochordate embryo revealed by single-blastomere transcriptome profiling.PLoS genetics · 2020Article
- Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish.Communications biology · 2020Article
- Functional equivalence of germ plasm organizers.PLoS genetics · 2018Article
- Modulation of F-actin dynamics by maternal Mid1ip1L controls germ plasm aggregation and furrow recruitment in the zebrafish embryo.Development (Cambridge, England) · 2018Article
- Workshop on Germ Cells.Frontiers in cell and developmental biology · 2018Article
- Naming CRISPR alleles: endonuclease-mediated mutation nomenclature across species.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Kinesin-1 promotes chondrocyte maintenance during skeletal morphogenesis.PLoS genetics · 2017Article
- Vertebrate Axial Patterning: From Egg to Asymmetry.Advances in experimental medicine and biology · 2017Review
- Methods to study maternal regulation of germ cell specification in zebrafish.Methods in cell biology · 2016Article
- Primordial Germ Cell Specification and Migration.F1000Research · 2015Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In animals, specification of the primordial germ cells (PGCs), the stem cells of the germ line, is required to transmit genetic information from one generation to the next. Bucky ball (Buc) is essential for germ plasm (GP) assembly in oocytes, and its overexpression results in excess PGCs in zebrafish embryos. However, the mechanistic basis for the excess PGCs in response to Buc overexpression, and whether endogenous Buc functions during embryogenesis, are unknown. Here, we show that endogenous Buc, like GP and overexpressed Buc-GFP, accumulates at embryonic cleavage furrows. Furthermore, we show that the maternally expressed zebrafish Kinesin-1 Kif5Ba is a binding partner of Buc and that maternal kif5Ba (Mkif5Ba) plays an essential role in germline specification in vivo. Specifically, Mkif5Ba is required to recruit GP to cleavage furrows and thereby specifies PGCs. Moreover, Mkif5Ba is required to enrich Buc at cleavage furrows and for the ability of Buc to promote excess PGCs, providing mechanistic insight into how Buc functions to assemble embryonic GP. In addition, we show that Mkif5Ba is also essential for dorsoventral (DV) patterning. Specifically, Mkif5Ba promotes formation of the parallel vegetal microtubule array required to asymmetrically position dorsal determinants (DDs) towards the prospective dorsal side. Interestingly, whereas Syntabulin and wnt8a translocation depend on kif5Ba, grip2a translocation does not, providing evidence for two distinct mechanisms by which DDs might be asymmetrically distributed. These studies identify essential roles for maternal Kif5Ba in PGC specification and DV patterning, and provide mechanistic insight into Buc functions during early embryogenesis.
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