ArticleMolecular & cellular proteomics : MCP2015
Post-Transcriptional Coordination of the Arabidopsis Iron Deficiency Response is Partially Dependent on the E3 Ligases RING DOMAIN LIGASE1 (RGLG1) and RING DOMAIN LIGASE2 (RGLG2).
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- TurboID-based proteomic profiling reveals proxitome of the IRT1 metal transporter and new insight into IRT1 endocytosis.The New phytologist · 2026Article
- Iron accumulation in leaf trichomes and stomata of tomato as an iron reservoir provided by iron-citric acid chelate under limey soil conditions.Biologia futura · 2025Article
- Transcriptome and translatome comparison of tissues from Arabidopsis thaliana.Scientific data · 2025Article
- Protein Phosphorylation Orchestrates Acclimations of Arabidopsis Plants to Environmental pH.Molecular & cellular proteomics : MCP · 2024Article
- PSI Photoinhibition and Changing COAntioxidants (Basel, Switzerland) · 2023Article
- The E3 ubiquitin ligase OsRGLG5 targeted by the Magnaporthe oryzae effector AvrPi9 confers basal resistance against rice blast.Plant communications · 2023Article
- L-DOPA induces iron accumulation in roots of Ipomoea aquatica and Arabidopsis thaliana in a pH-dependent manner.Botanical studies · 2023Article
- Endosomally Localized RGLG-Type E3 RING-Finger Ligases Modulate Sorting of Ubiquitylation-Mimic PIN2.International journal of molecular sciences · 2022Article
- Genomically Hardwired Regulation of Gene Activity Orchestrates Cellular Iron Homeostasis in Arabidopsis.RNA biology · 2022Article
- The Ubiquitin Proteasome System and Nutrient Stress Response.Frontiers in plant science · 2022Review
- IRONMAN tunes responses to iron deficiency in concert with environmental pH.Plant physiology · 2021Article
- Iron insufficiency in floral buds impairs pollen development by disrupting tapetum function.The Plant journal : for cell and molecular biology · 2021Article
- PRC2-mediated H3K27me3 modulates shoot iron homeostasis inPlant signaling & behavior · 2020Article
- Alteration of iron responsive gene expression in Arabidopsis glutaredoxinPlant signaling & behavior · 2020Article
- Regulation of Iron Homeostasis and Use in Chloroplasts.International journal of molecular sciences · 2020Review
- Scopoletin 8-Hydroxylase-Mediated Fraxetin Production Is Crucial for Iron Mobilization.Plant physiology · 2018Article
- OTU5 tunes environmental responses by sustaining chromatin structure.Plant signaling & behavior · 2018Article
- The Understanding of the Plant Iron Deficiency Responses in Strategy I Plants and the Role of Ethylene in This Process by Omic Approaches.Frontiers in plant science · 2017Review
- Systems-wide analysis of manganese deficiency-induced changes in gene activity of Arabidopsis roots.Scientific reports · 2016Article
- Transgenerational effects persist down the maternal line in marine sticklebacks: gene expression matches physiology in a warming ocean.Evolutionary applications · 2016Article
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acclimation to changing environmental conditions is mediated by proteins, the abundance of which is carefully tuned by an elaborate interplay of DNA-templated and post-transcriptional processes. To dissect the mechanisms that control and mediate cellular iron homeostasis, we conducted quantitative high-resolution iTRAQ proteomics and microarray-based transcriptomic profiling of iron-deficient Arabidopsis thaliana plants. A total of 13,706 and 12,124 proteins was identified with a quadrupole-Orbitrap hybrid mass spectrometer in roots and leaves, respectively. This deep proteomic coverage allowed accurate estimates of post-transcriptional regulation in response to iron deficiency. Similarly regulated transcripts were detected in only 13% (roots) and 11% (leaves) of the 886 proteins that differentially accumulated between iron-sufficient and iron-deficient plants, indicating that the majority of the iron-responsive proteins was post-transcriptionally regulated. Mutants harboring defects in the RING DOMAIN LIGASE1 (RGLG1)(1) and RING DOMAIN LIGASE2 (RGLG2) showed a pleiotropic phenotype that resembled iron-deficient plants with reduced trichome density and the formation of branched root hairs. Proteomic and transcriptomic profiling of rglg1 rglg2 double mutants revealed that the functional RGLG protein is required for the regulation of a large set of iron-responsive proteins including the coordinated expression of ribosomal proteins. This integrative analysis provides a detailed catalog of post-transcriptionally regulated proteins and allows the concept of a chiefly transcriptionally regulated iron deficiency response to be revisited. Protein data are available via ProteomeXchange with identifier PXD002126.
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