ArticleBMC urology2015
Localization of P2X receptor subtypes 2, 3 and 7 in human urinary bladder.
Article in BMC urology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Biomechanical regulation of bladder injury and repair.Nature reviews. Urology · 2026Review
- Interstitial Cells of Cajal and PJournal of clinical medicine · 2024Article
- Review
- The Pannexin 1 Channel and the P2X7 Receptor Are in Complex Interplay to Regulate the Release of Soluble Ectonucleotidases in the Murine Bladder Lamina Propria.International journal of molecular sciences · 2023Article
- Editorial: Novel mechanisms involved in urinary bladder control: Advances in neural, humoral and local factors underlying function and disease, volume II.Frontiers in physiology · 2022Article
- Protective Effect of Purinergic P2X7 Receptor Inhibition on Acrolein-Induced Urothelial Cell Damage.Frontiers in physiology · 2022Article
- Eliapixant is a selective P2X3 receptor antagonist for the treatment of disorders associated with hypersensitive nerve fibers.Scientific reports · 2021Article
- Analysis of Spatial and Temporal Distribution of Purinergic P2 Receptors in the Mouse Hippocampus.International journal of molecular sciences · 2021Article
- Immunohistochemistry as a paramount tool in research of normal urothelium, bladder cancer and bladder pain syndrome.European journal of histochemistry : EJH · 2021Review
- P2X7 Receptor Blockade Protects Against Acrolein-Induced Bladder Damage: A Potential New Therapeutic Approach for the Treatment of Bladder Inflammatory Diseases.Frontiers in pharmacology · 2021Article
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- The Role of Purinergic Receptors in the Circadian System.International journal of molecular sciences · 2020Review
- Purinergic receptor mediated calcium signalling in urothelial cells.Scientific reports · 2019Article
- The Botulinum Treatment of Neurogenic Detrusor Overactivity: The Double-Face of the Neurotoxin.Toxins · 2019Review
- Purinergic P2X7 receptors as therapeutic targets in interstitial cystitis/bladder pain syndrome; key role of ATP signaling in inflammation.Bladder (San Francisco, Calif.) · 2019Review
- The role of ATP signalling in response to mechanical stimulation studied in T24 cells using new microphysiological tools.Journal of cellular and molecular medicine · 2018Article
- TRPV1 enhances the afferent response to P2X receptor activation in the mouse urinary bladder.Scientific reports · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVoiding dysfunctions are a common problem that has a severe negative impact on the quality of life. Today there is a need for new drug targets for these conditions. The role of ATP receptors in bladder physiology has been studied for some time, primarily in animal models. The aim of this work is to investigate the localization of the ATP receptors P2X2, P2X3 and P2X7 and their colocalization with vimentin and actin in the human urinary bladder.
methodsImmunohistochemical analysis was conducted on full-thickness bladder tissues from fundus and trigonum collected from 15 patients undergoing open radical cystectomy due to chronic cystitis, bladder cancer or locally advanced prostate cancer. Colocalization analyses were performed between the three different P2X subtypes and the structural proteins vimentin and actin. Specimens were examined using epifluorescence microscopy and correlation coefficients were calculated for each costaining as well as the mean distance from the laminin positive basal side of the urothelium to the vimentin positive cells located in the suburothelium.
resultsP2X2 was expressed in vimentin positive cells located in the suburothelium. Less distinct labelling of P2X2 was also observed in actin positive smooth muscle cells and in the urothelium. P2X3 was expressed in vimentin positive cells surrounding the smooth muscle, and in vimentin positive cells located in the suburothelium. Weaker P2X3 labelling was seen in the urothelium. P2X7 was expressed in the smooth muscle cells and the urothelium. In the suburothelium, cells double positive for P2X2 and vimentin where located closer to the urothelium while cells double positive for P2X3 and vimentin where located further from the urothelium.
conclusionThe results from this study demonstrate that there is a significant difference in the expression of the purinergic P2X2, P2X3 and P2X7 receptors in the different histological layers of the human urinary bladder.
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