ArticlePLoS biology2015
Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex.
Article in PLoS biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 102 citations in OpenAlex.
- Direct and indirect neurogenesis from radial glial progenitor cell clones in the mouse neocortex.The EMBO journal · 2026Article
- Shaping the Neocortex: Radial Glia and Astrocytes in Development and Evolution.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Review
- A dyad of human-specificScience advances · 2025Article
- Indirect neurogenesis in space and time.Nature reviews. Neuroscience · 2024Review
- Transcriptional regulation in the development and dysfunction of neocortical projection neurons.Neural regeneration research · 2024Review
- H3 Acetylation-Induced Basal Progenitor Generation and Neocortex Expansion Depends on the Transcription Factor Pax6.Biology · 2024Article
- Reduced neural progenitor cell count and cortical neurogenesis in guinea pigs congenitally infected with Toxoplasma gondii.Communications biology · 2023Article
- Neocortex neurogenesis and maturation in the African greater cane rat.Neural development · 2023Article
- Human SERPINA3 induces neocortical folding and improves cognitive ability in mice.Cell discovery · 2022Article
- Pax6 limits the competence of developing cerebral cortical cells to respond to inductive intercellular signals.PLoS biology · 2022Article
- Evolution of genetic mechanisms regulating cortical neurogenesis.Developmental neurobiology · 2022Review
- Thirty Years' History since the Discovery of Pax6: From Central Nervous System Development to Neurodevelopmental Disorders.International journal of molecular sciences · 2022Review
- Review
- Regulation of Cell Delamination During Cortical Neurodevelopment and Implication for Brain Disorders.Frontiers in neuroscience · 2022Review
- Forebrain Organoids to Model the Cell Biology of Basal Radial Glia in Neurodevelopmental Disorders and Brain Evolution.Frontiers in cell and developmental biology · 2022Review
- Progenitor-Based Cell Biological Aspects of Neocortex Development and Evolution.Frontiers in cell and developmental biology · 2022Review
- Single cell multi-omic analysis identifies a Tbx1-dependent multilineage primed population in murine cardiopharyngeal mesoderm.Nature communications · 2021Article
- Inheritance and flexibility of cell polarity: a clue for understanding human brain development and evolution.Development (Cambridge, England) · 2021Article
- Expression of human-specific ARHGAP11B in mice leads to neocortex expansion and increased memory flexibility.The EMBO journal · 2021Article
- Dynamics of cortical progenitors and production of subcerebral neurons are altered in embryos of a maternal inflammation model for autism.Molecular psychiatry · 2021Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The evolutionary expansion of the neocortex in mammals has been linked to enlargement of the subventricular zone (SVZ) and increased proliferative capacity of basal progenitors (BPs), notably basal radial glia (bRG). The transcription factor Pax6 is known to be highly expressed in primate, but not mouse, BPs. Here, we demonstrate that sustaining Pax6 expression selectively in BP-genic apical radial glia (aRG) and their BP progeny of embryonic mouse neocortex suffices to induce primate-like progenitor behaviour. Specifically, we conditionally expressed Pax6 by in utero electroporation using a novel, Tis21-CreERT2 mouse line. This expression altered aRG cleavage plane orientation to promote bRG generation, increased cell-cycle re-entry of BPs, and ultimately increased upper-layer neuron production. Upper-layer neuron production was also increased in double-transgenic mouse embryos with sustained Pax6 expression in the neurogenic lineage. Strikingly, increased BPs existed not only in the SVZ but also in the intermediate zone of the neocortex of these double-transgenic mouse embryos. In mutant mouse embryos lacking functional Pax6, the proportion of bRG among BPs was reduced. Our data identify specific Pax6 effects in BPs and imply that sustaining this Pax6 function in BPs could be a key aspect of SVZ enlargement and, consequently, the evolutionary expansion of the neocortex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.