Evidence map›Paper›PMID 26252054›Full record

ArticleMolecular medicine reports2015

Lentivirus‑delivered nemo‑like kinase small interfering RNA inhibits laryngeal cancer cell proliferation in vitro.

Jun Tai, Yuansheng Rao, Jugao Fang, Zhigang Huang, Zhenkun Yu, Xiaohong Chen, Weiguo Zhou, Xiao Xiao, Ting Long, Yang Han and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Jun TaiBeijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
Yuansheng RaoDepartment of Otolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China.
Jugao FangKey Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
Zhigang HuangKey Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
Zhenkun YuKey Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
Xiaohong ChenKey Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
Weiguo ZhouKey Laboratory of Otolaryngology‑Head and Neck Surgery, Ministry of Education, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, P.R. China.
Xiao XiaoBeijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
Ting LongBeijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
Yang HanBeijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
Qiaoyin LiuBeijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
Aidong LiDepartment of Center Laboratory, Cancer Institute and Hospital, Chinese Academy of Medical Sciences, Beijing 100021, P.R. China.
Xin NiDepartment of Otolaryngology Head and Neck Surgery, Beijing Children's Hospital, Capital Medical University, Beijing 100045, P.R. China.
Capital Medical University · CNBeijing Tongren Hospital · CNBeijing Children’s Hospital · CNBeijing Anzhen Hospital · CNChinese Academy of Medical Sciences & Peking Union Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Laryngeal squamous cell carcinoma is the most common form of head and neck squamous cell carcinoma. Multiple approaches have been applied to treat this type of cancer; however, no significant improvement in survival rate has been achieved. In the present study, the role of nemo‑like kinase (NLK) in human laryngeal carcinoma Hep‑2 cells was investigated. NLK has been identified as an important regulator of cell growth, patterning and cell death in a variety of organisms. Lentivirus‑mediated‑shRNA was employed to silence endogenous NLK expression. Downregulation of the expression of NLK following lentivirus infection was confirmed using reverse transcription quantitative polymerase chain reaction and western blot analysis. The effects of NLK downregulation on Hep‑2 cell proliferation and cell cycle progression were analyzed using an MTT assay and flow cytometry, respectively. Downregulation of NLK also inhibited tumorigenesis and regulated the expression of cell cycle protein expression levels. Therefore, it was hypothesized that NLK is necessary for cell survival and tumorigenesis in laryngeal cancer cells. Furthermore, the absence of NLK may lead to cancer cell death. Collectively, the results of the present study demonstrated that the lentivirus‑mediated targeted disruption of NLK may be a promising therapeutic method for the treatment of laryngeal cancer.

Indexed as

Gene Expression Regulation, NeoplasticApoptosisCell Line, TumorCell MovementCell ProliferationCell Transformation, NeoplasticCyclin B1Cyclin D1Epithelial CellsGene SilencingGenetic VectorsHumansIntracellular Signaling Peptides and ProteinsLarynxLentivirusPhosphorylationCCNB1 protein, humanCCND1 protein, humanCyclin B1Cyclin D1Intracellular Signaling Peptides and ProteinsNLK protein, humanProtein Serine-Threonine KinasesRNA, Small InterferingTumor Suppressor Protein p53

Identifiers

PMID26252054
PMCPMC4581764
OpenAlexW2128692006

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.