Evidence map›Paper›PMID 26251254›Full record

ArticleStroke2015

Activation of Dopamine D2 Receptor Suppresses Neuroinflammation Through αB-Crystalline by Inhibition of NF-κB Nuclear Translocation in Experimental ICH Mice Model.

Yang Zhang, Yujie Chen, Jiang Wu, Anatol Manaenko, Peng Yang, Jiping Tang, Weiling Fu, John H Zhang

Expression of concernErratum issuedOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stroke, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 82 papers.

0numbers the graph read from it
0cells of the map it votes in
82citing papers in PubMed
8.7field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

82 citing papers in PubMed, 166 citations in OpenAlex.

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  15. Neuroprotective effect of ropinirole against AβThe Kaohsiung journal of medical sciences · 2023
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22 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Yang ZhangFrom the Department of Laboratory Medicine (Y.Z., W.F.) and Department of Neurosurgery (Y.C.), Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Physiology and Pharmacology, Loma Linda University, CA (Y.Z., Y.C., J.W., A.M., P.Y., J.T., J.H.Z.); and Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, China (J.W.).
Yujie ChenFrom the Department of Laboratory Medicine (Y.Z., W.F.) and Department of Neurosurgery (Y.C.), Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Physiology and Pharmacology, Loma Linda University, CA (Y.Z., Y.C., J.W., A.M., P.Y., J.T., J.H.Z.); and Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, China (J.W.).
Jiang WuFrom the Department of Laboratory Medicine (Y.Z., W.F.) and Department of Neurosurgery (Y.C.), Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Physiology and Pharmacology, Loma Linda University, CA (Y.Z., Y.C., J.W., A.M., P.Y., J.T., J.H.Z.); and Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, China (J.W.).
Anatol ManaenkoFrom the Department of Laboratory Medicine (Y.Z., W.F.) and Department of Neurosurgery (Y.C.), Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Physiology and Pharmacology, Loma Linda University, CA (Y.Z., Y.C., J.W., A.M., P.Y., J.T., J.H.Z.); and Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, China (J.W.).
Peng YangFrom the Department of Laboratory Medicine (Y.Z., W.F.) and Department of Neurosurgery (Y.C.), Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Physiology and Pharmacology, Loma Linda University, CA (Y.Z., Y.C., J.W., A.M., P.Y., J.T., J.H.Z.); and Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, China (J.W.).
Jiping TangFrom the Department of Laboratory Medicine (Y.Z., W.F.) and Department of Neurosurgery (Y.C.), Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Physiology and Pharmacology, Loma Linda University, CA (Y.Z., Y.C., J.W., A.M., P.Y., J.T., J.H.Z.); and Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, China (J.W.).
Weiling FuFrom the Department of Laboratory Medicine (Y.Z., W.F.) and Department of Neurosurgery (Y.C.), Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Physiology and Pharmacology, Loma Linda University, CA (Y.Z., Y.C., J.W., A.M., P.Y., J.T., J.H.Z.); and Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, China (J.W.). johnzhang3910@yahoo.com weilingfu8888@163.com.
John H ZhangFrom the Department of Laboratory Medicine (Y.Z., W.F.) and Department of Neurosurgery (Y.C.), Southwest Hospital, Third Military Medical University, Chongqing, China; Department of Physiology and Pharmacology, Loma Linda University, CA (Y.Z., Y.C., J.W., A.M., P.Y., J.T., J.H.Z.); and Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu Province, 215006, China (J.W.). johnzhang3910@yahoo.com weilingfu8888@163.com.
Army Medical University · CNFirst Affiliated Hospital of Soochow University · CNSouthwest Hospital · CN

Funding

Vascular Biology Core FacilityP01NS082184 · NINDS · LOMA LINDA UNIVERSITY · PI PEARCE, WILLIAM J. · 2014 to 2018
$6.1M
NINDS NIH HHS NS082184NINDS NIH HHS P01 NS082184
6 · The paper itself

Abstract

background and purposeInflammatory injury plays a critical role in intracerebral hemorrhage (ICH)-induced secondary brain injury. Recently, dopamine D2 receptor (DRD2) is identified as an important component controlling innate immunity and inflammatory response in central nervous system, and αB-crystallin (CRYAB) is a potent negative regulator on inflammatory pathways. Here, we sought to investigate the role of DRD2 on neuroinflammation after experimental ICH and the potential mechanism mediated by CRYAB.

methodsTwo hundred and twenty-four (224) male CD-1 mice were subjected to intrastriatal infusion of bacterial collagenase or autologous blood. Two DRD2 agonists quinpirole and ropinirole were administrated by daily intraperitoneal injection starting at 1 hour after ICH. DRD2 and CRYAB in vivo knockdown was performed 48 hours before ICH insult. Behavioral deficits and brain water content, Western blots, immunofluorescence staining, coimmunoprecipitation (Co-IP) assay, and proteome cytokine array were evaluated.

resultsEndogenous DRD2 and CRYAB expressions were increased after ICH. DRD2 knockdown aggravated the neurobehavioral deficits and the pronounced cytokine expressions. DRD2 activation by quinpirole and ropinirole ameliorated neurological outcome, brain edema, interleukin-1β, and monocyte chemoattractant protein-1 expression, as well as microglia/macrophages activation, in the perihematomal region. These effects were abolished by pretreatment with CRYAB siRNAs. Quinpirole enhanced cytoplasmic binding activity between CRYAB and NF-κB and decreased nuclear NF-κB expression. Similar therapeutic benefits were observed using autologous blood injection model and intranasal delivery of quinpirole.

conclusionsDRD2 may have anti-inflammatory effects after ICH. DRD2 agonists inhibited neuroinflammation and attenuated brain injury after ICH, which is probably mediated by CRYAB and enhanced cytoplasmic binding activity with NF-κB.

Indexed as

Active Transport, Cell Nucleusalpha-Crystallin B ChainAnimalsCell NucleusCerebral HemorrhageDisease Models, AnimalInflammationMaleMiceMice, Inbred StrainsNF-kappa BReceptors, Dopamine D2alpha-Crystallin B ChainNF-kappa BReceptors, Dopamine D2B-crystallinedopamine D2 receptorintracerebral hemorrhageneuroinflammationNF-κB

Identifiers

PMID26251254
PMCPMC4550518
OpenAlexW2146037555

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.